The following data files are provided: • I2 & A2: These are .tif files containing binarised Micro-CT image stacks for scaffolds I2 and A2 respectively. The image stack is 550 pixels across in all dimensions, where 1 pixel = 3.74 µm. NB: Corresponding .tif files for scaffolds I1 and A1 are available as supporting data at https://www.repository.cam.ac.uk/handle/1810/252372. • Real-time videos: A .zip file containing the movies of single cell migration in each scaffold, from which cell velocity was measured. Files for scaffolds A1 and A2 are labelled as “isotropic” or “anisotropic” according to whether the image plane contains long range pore wall alignment. • Invasion data: A .zip file containing the fluorescent and corresponding bright field images of each scaffold cross-section at day 3 and day 7 after seeding. • Collated data: An excel file containing the measurements of pore size and percolation diameter taken from the Micro-CT files listed above, cell velocities measured from the movies listed above, and cell position/orientation index measured from the fluorescent/bright field images listed above.