Systematic longitudinal survey of invasive Escherichia coli in England demonstrates a stable population structure only transiently disturbed by the emergence of ST131
Authors
Kallonen, TT
Brodrick, H
Harris, S
Corander, J
Brown, N
Martin, V
Publication Date
2017-07-18Journal Title
Genome Research
ISSN
1088-9051
Publisher
Cold Spring Harbor Laboratory Press
Volume
27
Pages
1437-1449
Language
English
Type
Article
This Version
AM
Metadata
Show full item recordCitation
Kallonen, T., Brodrick, H., Harris, S., Corander, J., Brown, N., Martin, V., Peacock, S., & et al. (2017). Systematic longitudinal survey of invasive Escherichia coli in England demonstrates a stable population structure only transiently disturbed by the emergence of ST131. Genome Research, 27 1437-1449. https://doi.org/10.1101/gr.216606.116
Abstract
$\textit{Escherichia coli}$ associated with urinary tract infections and bacteremia has been intensively investigated, including recent work focusing on the virulent, globally disseminated, multidrug-resistant lineage ST131. To contextualize ST131 within the broader $\textit{E. coli}$ population associated with disease, we used genomics to analyze a systematic 11-yr hospital-based survey of $\textit{E. coli}$ associated with bacteremia using isolates collected from across England by the British Society for Antimicrobial Chemotherapy and from the Cambridge University Hospitals NHS Foundation Trust. Population dynamics analysis of the most successful lineages identified the emergence of ST131 and ST69 and their establishment as two of the five most common lineages along with ST73, ST95, and ST12. The most frequently identified lineage was ST73. Compared to ST131, ST73 was susceptible to most antibiotics, indicating that multidrug resistance was not the dominant reason for prevalence of $\textit{E. coli}$ lineages in this population. Temporal phylogenetic analysis of the emergence of ST69 and ST131 identified differences in the dynamics of emergence and showed that expansion of ST131 in this population was not driven by sequential emergence of increasingly resistant subclades. We showed that over time, the $\textit{E. coli}$ population was only transiently disturbed by the introduction of new lineages before a new equilibrium was rapidly achieved. Together, these findings suggest that the frequency of $\textit{E. coli}$ lineages in invasive disease is driven by negative frequency-dependent selection occurring outside of the hospital, most probably in the commensal niche, and that drug resistance is not a primary determinant of success in this niche.
Sponsorship
This publication presents independent research supported by the Health Innovation Challenge Fund (HICF-T5-342 and WT098600), a parallel funding partnership between the UK Department of Health and Wellcome Trust. The views expressed in this publication are those of the authors and not necessarily those of the Department of Health, Public Health England or the Wellcome Trust.
Funder references
Wellcome Trust (098600/Z/12/Z)
Embargo Lift Date
2100-01-01
Identifiers
External DOI: https://doi.org/10.1101/gr.216606.116
This record's URL: https://www.repository.cam.ac.uk/handle/1810/265380
Rights
Attribution 4.0 International, Attribution 4.0 International, Attribution 4.0 International, Attribution 4.0 International