File formatt is .tiff, with each .tiff file representing a single frame of video or slice of stack. Individual vesicles are organised into .zip files corresponding to their SA/DNA concentration ratios and experiment type: Z-Stack, Flickering or FRAP. In the Z-stacks .zip files, two channels were recorded, using a 488nm and 633nm laser respectively. For each vesicle folder, there is a separate subfolder for each of these channels channel. Each frame of the Z-stacks represents a 0.1um slice. In Flickering videos, each .tiff file is a frame of a 1000 frame video, with a framerate of 97ms/frame, taken at the equator of the vesicle. In FRAP experiments, each vesicle subfolder contains subfolders at different frame rates. These are: StagePre (10fps), StagePost1 (10fps), StagePost2 (0.5fps) and StagePost3 (0.1fps). These correspond to stages of the pre- and post-bleaching process.