A phagocytic route for uptake of double-stranded RNA in RNAi.
Rocha, João JE
Korolchuk, Viktor I
Robinson, Iain M
O'Kane, Cahir J
Public Library of Science (PLoS)
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Rocha, J. J., Korolchuk, V. I., Robinson, I. M., & O'Kane, C. J. (2011). A phagocytic route for uptake of double-stranded RNA in RNAi.. PLoS One, 6 (4), e19087. https://doi.org/10.1371/journal.pone.0019087
RNA interference (RNAi) has a range of physiological functions including as a defence mechanism against viruses. To protect uninfected cells in a multicellular organism, not only a cell-autonomous RNAi response is required but also a systemic one. However, the route of RNA spread in systemic RNAi remains unclear. Here we show that phagocytosis can be a route for double-stranded RNA uptake. Double-stranded RNA expressed in Escherichia coli induces robust RNAi in Drosophila S2 cells, with effectiveness comparable to that of naked dsRNA. We could separate this phagocytic uptake route from that for RNAi induced by naked dsRNA. Therefore, phagocytic uptake of dsRNA offers a potential route for systemic spread of RNAi.
Cell Line, Animals, Drosophila melanogaster, Escherichia coli, Cytochalasin D, Ribonuclease III, Carrier Proteins, Drosophila Proteins, Clathrin, RNA, Double-Stranded, Nucleic Acid Synthesis Inhibitors, Endocytosis, Phagocytosis, RNA Interference, Base Sequence, Molecular Sequence Data
Biotechnology and Biological Sciences Research Council (BB/D019699/1)
External DOI: https://doi.org/10.1371/journal.pone.0019087
This record's URL: https://www.repository.cam.ac.uk/handle/1810/280179
Attribution 4.0 International
Licence URL: https://creativecommons.org/licenses/by/4.0/