A phagocytic route for uptake of double-stranded RNA in RNAi.
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Authors
Rocha, João JE
Korolchuk, Viktor I
Robinson, Iain M
O'Kane, Cahir J
Publication Date
2011-04-29Journal Title
PLoS One
ISSN
1932-6203
Publisher
Public Library of Science (PLoS)
Volume
6
Issue
4
Pages
e19087
Language
eng
Type
Article
Physical Medium
Electronic
Metadata
Show full item recordCitation
Rocha, J. J., Korolchuk, V. I., Robinson, I. M., & O'Kane, C. J. (2011). A phagocytic route for uptake of double-stranded RNA in RNAi.. PLoS One, 6 (4), e19087. https://doi.org/10.1371/journal.pone.0019087
Abstract
RNA interference (RNAi) has a range of physiological functions including as a defence mechanism against viruses. To protect uninfected cells in a multicellular organism, not only a cell-autonomous RNAi response is required but also a systemic one. However, the route of RNA spread in systemic RNAi remains unclear. Here we show that phagocytosis can be a route for double-stranded RNA uptake. Double-stranded RNA expressed in Escherichia coli induces robust RNAi in Drosophila S2 cells, with effectiveness comparable to that of naked dsRNA. We could separate this phagocytic uptake route from that for RNAi induced by naked dsRNA. Therefore, phagocytic uptake of dsRNA offers a potential route for systemic spread of RNAi.
Keywords
Cell Line, Animals, Drosophila melanogaster, Escherichia coli, Cytochalasin D, Ribonuclease III, Carrier Proteins, Drosophila Proteins, Clathrin, RNA, Double-Stranded, Nucleic Acid Synthesis Inhibitors, Endocytosis, Phagocytosis, RNA Interference, Base Sequence, Molecular Sequence Data
Sponsorship
Biotechnology and Biological Sciences Research Council (BB/D019699/1)
Identifiers
External DOI: https://doi.org/10.1371/journal.pone.0019087
This record's URL: https://www.repository.cam.ac.uk/handle/1810/280179
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