Recognizing the reagent microbiome
Accepted version
Peer-reviewed
Repository URI
Repository DOI
Change log
Authors
Lager, Susanne
Wagner, Josef
Abstract
A noticeable part of the microbiome literature, especially that working with low-biomass samples, is plagued by reagent contamination. Here we describe visual, statistical, methodical and ecological techniques to facilitate recognition of signals which represent contamination.
Description
Keywords
DNA Contamination, Databases, Genetic, Gastrointestinal Microbiome, High-Throughput Nucleotide Sequencing, Humans, Indicators and Reagents, Microbiota, RNA, Ribosomal, 16S
Journal Title
Nature Reviews Microbiology
Conference Name
Journal ISSN
1740-1526
2058-5276
2058-5276
Volume Title
3
Publisher
Springer Nature
Publisher DOI
Sponsorship
Medical Research Council (MR/K021133/1)
Cambridge University Hospitals NHS Foundation Trust (CUH) (146281)
Medical Research Council (G1100221)
Medical Research Council (G1100221/1)
Cambridge University Hospitals NHS Foundation Trust (CUH) (146281)
Medical Research Council (G1100221)
Medical Research Council (G1100221/1)
The work was supported by the Medical Research Council (United Kingdom; G1100221) and the National Institute for Health Research (NIHR) Cambridge Comprehensive Biomedical Research Centre (Women’s Health theme).