Recognizing the reagent microbiome

Authors
Lager, Susanne 
Wagner, Josef 
Kronbichler, Andreas  ORCID logo  https://orcid.org/0000-0002-2945-2946
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DOI
https://doi.org/10.1038/s41564-018-0202-y
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Accepted version (40.32 KB)
Supporting information (5.74 MB)
Type
Article
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Abstract

A noticeable part of the microbiome literature, especially that working with low-biomass samples, is plagued by reagent contamination. Here we describe visual, statistical, methodical and ecological techniques to facilitate recognition of signals which represent contamination.

Publication Date
2018-08
Online Publication Date
2018-07-25
Acceptance Date
2018-04-27
Keywords
DNA Contamination, Databases, Genetic, Gastrointestinal Microbiome, High-Throughput Nucleotide Sequencing, Humans, Indicators and Reagents, Microbiota, RNA, Ribosomal, 16S
Journal Title
Nature Reviews Microbiology
Journal ISSN
1740-1526
2058-5276
Volume Title
3
Publisher
Springer Nature
Rights
http://www.rioxx.net/licenses/all-rights-reserved
Sponsorship
Medical Research Council (MR/K021133/1)
Cambridge University Hospitals NHS Foundation Trust (CUH) (146281)
Medical Research Council (G1100221)
The work was supported by the Medical Research Council (United Kingdom; G1100221) and the National Institute for Health Research (NIHR) Cambridge Comprehensive Biomedical Research Centre (Women’s Health theme).
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Cambridge University Research Outputs