Recognizing the reagent microbiome
Authors
Lager, Susanne
Wagner, Josef
Change log
Abstract
A noticeable part of the microbiome literature, especially that working with low-biomass samples, is plagued by reagent contamination. Here we describe visual, statistical, methodical and ecological techniques to facilitate recognition of signals which represent contamination.
Publication Date
2018-08
Online Publication Date
2018-07-25
Acceptance Date
2018-04-27
Keywords
DNA Contamination, Databases, Genetic, Gastrointestinal Microbiome, High-Throughput Nucleotide Sequencing, Humans, Indicators and Reagents, Microbiota, RNA, Ribosomal, 16S
Journal Title
Nature Reviews Microbiology
Journal ISSN
1740-1526
2058-5276
2058-5276
Volume Title
3
Publisher
Springer Nature
Sponsorship
Medical Research Council (MR/K021133/1)
Cambridge University Hospitals NHS Foundation Trust (CUH) (146281)
Medical Research Council (G1100221)
Cambridge University Hospitals NHS Foundation Trust (CUH) (146281)
Medical Research Council (G1100221)
The work was supported by the Medical Research Council (United Kingdom; G1100221) and the National Institute for Health Research (NIHR) Cambridge Comprehensive Biomedical Research Centre (Women’s Health theme).