Integration of hormonal signaling networks and mobile microRNAs is required for vascular patterning in Arabidopsis roots.
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Authors
Muraro, Daniele
Mellor, Nathan
Pound, Michael P
Help, Hanna
Lucas, Mikaël
Chopard, Jérôme
Byrne, Helen M
Godin, Christophe
Hodgman, T Charlie
King, John R
Pridmore, Tony P
Bennett, Malcolm J
Bishopp, Anthony
Publication Date
2014-01-14Journal Title
Proc Natl Acad Sci U S A
ISSN
0027-8424
Publisher
Proceedings of the National Academy of Sciences
Volume
111
Issue
2
Pages
857-862
Language
eng
Type
Article
Physical Medium
Print-Electronic
Metadata
Show full item recordCitation
Muraro, D., Mellor, N., Pound, M. P., Help, H., Lucas, M., Chopard, J., Byrne, H. M., et al. (2014). Integration of hormonal signaling networks and mobile microRNAs is required for vascular patterning in Arabidopsis roots.. Proc Natl Acad Sci U S A, 111 (2), 857-862. https://doi.org/10.1073/pnas.1221766111
Abstract
As multicellular organisms grow, positional information is continually needed to regulate the pattern in which cells are arranged. In the Arabidopsis root, most cell types are organized in a radially symmetric pattern; however, a symmetry-breaking event generates bisymmetric auxin and cytokinin signaling domains in the stele. Bidirectional cross-talk between the stele and the surrounding tissues involving a mobile transcription factor, SHORT ROOT (SHR), and mobile microRNA species also determines vascular pattern, but it is currently unclear how these signals integrate. We use a multicellular model to determine a minimal set of components necessary for maintaining a stable vascular pattern. Simulations perturbing the signaling network show that, in addition to the mutually inhibitory interaction between auxin and cytokinin, signaling through SHR, microRNA165/6, and PHABULOSA is required to maintain a stable bisymmetric pattern. We have verified this prediction by observing loss of bisymmetry in shr mutants. The model reveals the importance of several features of the network, namely the mutual degradation of microRNA165/6 and PHABULOSA and the existence of an additional negative regulator of cytokinin signaling. These components form a plausible mechanism capable of patterning vascular tissues in the absence of positional inputs provided by the transport of hormones from the shoot.
Keywords
Arabidopsis, Plant Roots, Plant Growth Regulators, Homeodomain Proteins, Arabidopsis Proteins, Transcription Factors, MicroRNAs, Microscopy, Confocal, Signal Transduction, Models, Biological, Plant Vascular Bundle
Identifiers
External DOI: https://doi.org/10.1073/pnas.1221766111
This record's URL: https://www.repository.cam.ac.uk/handle/1810/284140
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