© 2018 The Combustion Institute. Biodiesel and blends with petroleum diesel have shown their potential as renewable alternative fuels for engines, with additional benefits of low particulate matter and low sulfate emissions. In this paper we measure the soot volume fraction produced by three different methyl esters processed biodiesels (extracted from palm (PME), soy (SME) and coconut (CME)), and their blends with petroleum diesel, in a series of co-flow stabilized laminar pool flames, using laser induced-incandescence (LII) and laser extinction optical methods. The soot volume fraction measurement results show that all neat biodiesels produce only up to 33% of the total soot volume compared to pure diesel, and that the total soot volume correlates directly with the degree of unsaturation of the biodiesels. Blending leads to approximately linear behaviour of total soot volume, with a shift in slope with smaller sensitivity towards neat diesel.