Show simple item record

dc.contributor.authorKirschner, Sandra
dc.contributor.authorWoodfield, Helen
dc.contributor.authorPrusko, Katharina
dc.contributor.authorKoczor, Maria
dc.contributor.authorGowik, Udo
dc.contributor.authorHibberd, Julian
dc.contributor.authorWesthoff, Peter
dc.date.accessioned2018-11-01T14:01:47Z
dc.date.available2018-11-01T14:01:47Z
dc.date.issued2018-09-14
dc.identifier.issn0022-0957
dc.identifier.urihttps://www.repository.cam.ac.uk/handle/1810/284473
dc.description.abstractThe bundle sheath provides a conduit linking veins and mesophyll cells. In the C3 plant Arabidopsis thaliana, it also plays important roles in oxidative stress and sulphur metabolism. However, the mechanisms responsible for the patterns of gene expression that underpin these metabolic specializations are poorly understood. Here, we used the Arabidopsis SULTR2;2 gene as a model to better understand mechanisms that restrict expression to the bundle sheath. Deletion analysis indicated that the SULTR2;2 promoter contains a short region necessary for expression in the bundle sheath and veins. This sequence acts as a positive regulator and is tolerant to multiple consecutive deletions indicating considerable redundancy in the cis-elements involved. It is highly conserved in SULTR2;2 genes of the Brassicaceae and is functional in the distantly related C4 species Flaveria bidentis that belongs to the Asteraceae. We conclude that expression of SULTR2;2 in the bundle sheath and veins is underpinned by a highly redundant sequence that likely represents an ancient and conserved mechanism found in families as diverse as the Asteraceae and Brassicaceae.
dc.description.sponsorshipBBSRC PhD studentship Deutsche Forschungsgemeinschaft
dc.format.mediumPrint
dc.languageeng
dc.publisherOxford University Press (OUP)
dc.rightsAttribution 4.0 International
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/
dc.subjectArabidopsis
dc.subjectPlant Leaves
dc.subjectSequence Alignment
dc.subjectGene Expression Regulation, Plant
dc.subjectBase Sequence
dc.subjectPlant Vascular Bundle
dc.titleExpression of SULTR2;2, encoding a low-affinity sulphur transporter, in the Arabidopsis bundle sheath and vein cells is mediated by a positive regulator.
dc.typeArticle
prism.endingPage4906
prism.issueIdentifier20
prism.publicationDate2018
prism.publicationNameJ Exp Bot
prism.startingPage4897
prism.volume69
dc.identifier.doi10.17863/CAM.31849
dcterms.dateAccepted2018-07-10
rioxxterms.versionofrecord10.1093/jxb/ery263
rioxxterms.licenseref.urihttp://www.rioxx.net/licenses/all-rights-reserved
rioxxterms.licenseref.startdate2018-09
dc.contributor.orcidHibberd, Julian [0000-0003-0662-7958]
dc.identifier.eissn1460-2431
rioxxterms.typeJournal Article/Review
cam.issuedOnline2018-07-19


Files in this item

Thumbnail

This item appears in the following Collection(s)

Show simple item record

Attribution 4.0 International
Except where otherwise noted, this item's licence is described as Attribution 4.0 International