Single-cell transcriptomes from human kidneys reveal the cellular identity of renal tumors.
Loudon, Kevin W
Armitage, James N
Riddick, Antony CP
Science (New York, N.Y.)
MetadataShow full item record
Young, M. D., Mitchell, T., Vieira Braga, F. A., Tran, M. G., Stewart, B. J., Ferdinand, J., Collord, G., et al. (2018). Single-cell transcriptomes from human kidneys reveal the cellular identity of renal tumors.. Science (New York, N.Y.), 361 (6402), 594-599. https://doi.org/10.1126/science.aat1699
Messenger RNA encodes cellular function and phenotype. In the context of human cancer it defines the identity of malignant cells and captures the diversity of tumor tissue. We sought to reveal the cellular identity and composition of kidney tumors, by studying 72,501 single cell transcriptomes of human renal tumors and normal tissue from fetal, pediatric and adult kidneys. We were able to match childhood Wilms’ tumor with specific fetal cell types, thus providing direct evidence for the hypothesis that Wilms’ tumor cells are aberrant fetal cells. In adult renal cell carcinoma we identified a canonical cancer transcriptome that matched a little known subtype of proximal convoluted tubular cell. Analyses of the tumor composition identified consistent preservation of cancer-associated mesangial cells, fibroblasts and a distorted vascular endothelial compartment. Our findings reveal the precise cellular identity and composition of human kidney tumors.
Kidney, Humans, Carcinoma, Renal Cell, Kidney Neoplasms, Adult, Child, Wilms Tumor, Genetic Variation, Single-Cell Analysis, Transcriptome
Wellcome Trust (096956/Z/11/Z)
MEDICAL RESEARCH COUNCIL (MR/M008975/1)
Department of Health (via National Institute for Health Research (NIHR)) (NF-SI-0514-10122)
WELLCOME TRUST (200110/Z/15/Z)
Arthritis Research UK (21777)
External DOI: https://doi.org/10.1126/science.aat1699
This record's URL: https://www.repository.cam.ac.uk/handle/1810/285014