Daily Onset of Light and Darkness Differentially Controls Hematopoietic Stem Cell Differentiation and Maintenance.
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Authors
Golan, Karin
Kumari, Anju
Kollet, Orit
Khatib-Massalha, Eman
Subramaniam, Mohana Devi
Avemaria, Francesca
García-García, Andrés
Itkin, Tomer
Ludin-Tal, Aya
Massalha, Hassan
Bernshtein, Biana
Brandis, Alexander
Mehlman, Tevie
Bhattacharya, Suditi
Bertagna, Mayla
Cheng, Hui
Petrovich-Kopitman, Ekaterina
Janus, Tomasz
Kaushansky, Nathali
Cheng, Tao
Sagi, Irit
Dick, John E
Markus, Regina P
Lapidot, Tsvee
Publication Date
2018-10-04Journal Title
Cell Stem Cell
ISSN
1934-5909
Publisher
Elsevier BV
Volume
23
Issue
4
Pages
572-585.e7
Language
eng
Type
Article
Physical Medium
Print-Electronic
Metadata
Show full item recordCitation
Golan, K., Kumari, A., Kollet, O., Khatib-Massalha, E., Subramaniam, M. D., Ferreira, Z. S., Avemaria, F., et al. (2018). Daily Onset of Light and Darkness Differentially Controls Hematopoietic Stem Cell Differentiation and Maintenance.. Cell Stem Cell, 23 (4), 572-585.e7. https://doi.org/10.1016/j.stem.2018.08.002
Abstract
Hematopoietic stem and progenitor cells (HSPCs) tightly couple maintenance of the bone marrow (BM) reservoir, including undifferentiated long-term repopulating hematopoietic stem cells (LT-HSCs), with intensive daily production of mature leukocytes and blood replenishment. We found two daily peaks of BM HSPC activity that are initiated by onset of light and darkness providing this coupling. Both peaks follow transient elevation of BM norepinephrine and TNF secretion, which temporarily increase HSPC reactive oxygen species (ROS) levels. Light-induced norepinephrine and TNF secretion augments HSPC differentiation and increases vascular permeability to replenish the blood. In contrast, darkness-induced TNF increases melatonin secretion to drive renewal of HSPCs and LT-HSC potential through modulating surface CD150 and c-Kit expression, increasing COX-2/αSMA+ macrophages, diminishing vascular permeability, and reducing HSPC ROS levels. These findings reveal that light- and darkness-induced daily bursts of norepinephrine, TNF, and melatonin within the BM are essential for synchronized mature blood cell production and HSPC pool repopulation.
Keywords
TNF, bone marrow, differentiation and egress, hematopoietic stem and progenitor cells, light and darkness, maintenance and retention, melatonin, norepinephrine, stem cell repopulation potential, vascular permeability, Animals, Cell Differentiation, Cells, Cultured, Darkness, Epigenesis, Genetic, Hematopoietic Stem Cells, Light, Mice, Mice, Inbred C57BL, Transcription Factors
Sponsorship
European Research Council (648765)
NHS Blood and Transplant (NHSBT)
Medical Research Council (MC_PC_12009)
Identifiers
External DOI: https://doi.org/10.1016/j.stem.2018.08.002
This record's URL: https://www.repository.cam.ac.uk/handle/1810/285544
Rights
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http://www.rioxx.net/licenses/all-rights-reserved
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