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dc.contributor.authorBeccari, Leonardo
dc.contributor.authorMoris, Naomi
dc.contributor.authorGirgin, Mehmet
dc.contributor.authorTurner, David A
dc.contributor.authorBaillie-Johnson, Peter
dc.contributor.authorCossy, Anne-Catherine
dc.contributor.authorLutolf, Matthias P
dc.contributor.authorDuboule, Denis
dc.contributor.authorArias, Alfonso Martinez
dc.date.accessioned2018-11-24T00:31:45Z
dc.date.available2018-11-24T00:31:45Z
dc.date.issued2018-10
dc.identifier.issn0028-0836
dc.identifier.urihttps://www.repository.cam.ac.uk/handle/1810/285960
dc.description.abstractThe emergence of multiple axes is an essential element in the establishment of the mammalian body plan. This process takes place shortly after implantation of the embryo within the uterus and relies on the activity of gene regulatory networks that coordinate transcription in space and time. Whereas genetic approaches have revealed important aspects of these processes1, a mechanistic understanding is hampered by the poor experimental accessibility of early post-implantation stages. Here we show that small aggregates of mouse embryonic stem cells (ESCs), when stimulated to undergo gastrulation-like events and elongation in vitro, can organize a post-occipital pattern of neural, mesodermal and endodermal derivatives that mimic embryonic spatial and temporal gene expression. The establishment of the three major body axes in these 'gastruloids'2,3 suggests that the mechanisms involved are interdependent. Specifically, gastruloids display the hallmarks of axial gene regulatory systems as exemplified by the implementation of collinear Hox transcriptional patterns along an extending antero-posterior axis. These results reveal an unanticipated self-organizing capacity of aggregated ESCs and suggest that gastruloids could be used as a complementary system to study early developmental events in the mammalian embryo.
dc.description.sponsorshipThis work was supported by funds from the BBSRC (No. BB/M023370/1 and BB/P003184/1 to A.M.A.), an NC3Rs David Sainsbury Fellowship (No. NC/P001467/1 to D.A.T.), an Engineering and Physical Sciences Research Council (EPSRC) Studentship (to P.B.-J.), a Company of Biologists Development Travelling Fellowship (DEVTF-151210 to P.B.-J.), a Newnham College Constance Work Junior Research Fellowship (to N.M.), the École Polytechnique Fédérale de Lausanne (D.D. and M.P.L.), the University of Geneva (D.D.), the Swiss National Research Fund (No. 310030B_138662 to D.D.) and the European Research Council grants ERC MOFDH (No. 250316 to A.M.A.), RegulHox (No 588029 to D.D.) and STEMCHIP (No 311422 to M.P.L.).
dc.languageeng
dc.publisherSpringer Nature
dc.titleMulti-axial self-organization properties of mouse embryonic stem cells into gastruloids.
dc.typeArticle
prism.endingPage276
prism.issueIdentifier7726
prism.publicationNameNature
prism.startingPage272
prism.volume562
dc.identifier.doi10.17863/CAM.33287
dcterms.dateAccepted2018-08-13
rioxxterms.versionofrecord10.1038/s41586-018-0578-0
rioxxterms.licenseref.urihttp://www.rioxx.net/licenses/all-rights-reserved
rioxxterms.licenseref.startdate2018-08-13
dc.contributor.orcidMoris, Naomi [0000-0003-1910-5454]
dc.contributor.orcidTurner, David [0000-0002-3447-7662]
dc.contributor.orcidBaillie-Johnson, Peter [0000-0003-2157-5017]
dc.contributor.orcidMartinez-Arias, Alfonso [0000-0002-1781-564X]
dc.identifier.eissn1476-4687
rioxxterms.typeJournal Article/Review
pubs.funder-project-idNational Centre for the Replacement Refinement and Reduction of Animals in Research (NC/P001467/1)
pubs.funder-project-idBiotechnology and Biological Sciences Research Council (BB/P003184/1)
pubs.funder-project-idEuropean Commission FP7 ERC Advanced Investigator Grants (AIG) (250316)
pubs.funder-project-idEPSRC (1359454)
pubs.funder-project-idEngineering and Physical Sciences Research Council (EP/K503009/1)
pubs.funder-project-idEngineering and Physical Sciences Research Council (EP/L504920/1)
cam.issuedOnline2018-10-03
rioxxterms.freetoread.startdate2019-10-03


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