The effect of succinate on brain NADH/NAD+ redox state and high energy phosphate metabolism in acute traumatic brain injury
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Authors
Stovell, MG
Carpenter, thomas
Thelin, eric
Yan, Jiun
Guilfoyle, Mathew
Jalloh, I
Howe, Duncan J
Grice, peter
Mason, Andrew
Giorgi-Coll, S
Gallagher, Clare N
Murphy, Michael
Menon, DK
Hutchinson, peter
Carpenter, keri
Publication Date
2018-07-24Journal Title
Scientific Reports
ISSN
2045-2322
Publisher
Nature Publishing Group
Volume
8
Number
11140
Type
Article
This Version
VoR
Metadata
Show full item recordCitation
Stovell, M., Mada, M., Helmy, A., Carpenter, t., Thelin, e., Yan, J., Guilfoyle, M., et al. (2018). The effect of succinate on brain NADH/NAD+ redox state and high energy phosphate metabolism in acute traumatic brain injury. Scientific Reports, 8 (11140) https://doi.org/10.1038/s41598-018-29255-3
Abstract
A key pathophysiological process and therapeutic target in the critical early post-injury period of traumatic brain injury (TBI) is cell mitochondrial dysfunction; characterised by elevation of brain lactate/pyruvate (L/P) ratio in the absence of hypoxia. We previously showed that succinate can improve brain extracellular chemistry in acute TBI, but it was not clear if this translates to a change in downstream energy metabolism. We studied the effect of microdialysis-delivered succinate on brain energy state (phosphocreatine/ATP ratio (PCr/ATP)) with 31P MRS at 3T, and tissue NADH/NAD+ redox state using microdialysis (L/P ratio) in eight patients with acute major TBI (mean 7 days). Succinate perfusion was associated with increased extracellular pyruvate (+26%, p <0.0001) and decreased L/P ratio ( 13%, p <0.0001) in patients overall (baseline-vs-supplementation over time), but no clear-cut change in 31P MRS PCr/ATP in our cohort (p >0.4, supplemented voxel-vs-contralateral voxel). However, the percentage decrease in L/P ratio for each patient following succinate perfusion correlated significantly with their percentage increase in PCr/ATP ratio (Spearman's rank correlation, r =-0.86, p =0.024). Our findings support the interpretation that L/P ratio is linked to brain energy state, and that succinate may support brain energy metabolism in select TBI patients suffering from mitochondrial dysfunction.
Keywords
Brain injuries, Molecular neuroscience, Neurochemistry, Translational research, Trauma
Sponsorship
MRC Grant No. G1002277 ID98489.
MRC Grant No. G0600986 ID79068.
MRC, MC_U105663142.
Wellcome Trust 110159/Z/15/Z.
National Institute for Health Research Biomedical Research Centre, Cambridge (Neuroscience Theme; Brain Injury and Repair Theme.
Medical Research Council Project code SRAG/071, ID RG87629.
Swedish Society for Medical Research.
Canadian Institute of Health Research
Medical Research Council/Royal College of Surgeons of England Clinical Research Training Fellowship Grant no. G0802251.
Funder references
TCC (None)
Medical Research Council (G1002277)
Medical Research Council (G0600986)
Wellcome Trust (110159/Z/15/Z)
Medical Research Council (MC_UU_00015/3)
Medical Research Council (MC_U105663142)
Medical Research Council (G0802251)
Medical Research Council (MR/N025792/1)
Identifiers
External DOI: https://doi.org/10.1038/s41598-018-29255-3
This record's URL: https://www.repository.cam.ac.uk/handle/1810/286016
Rights
Attribution 4.0 International, Attribution 4.0 International
Licence URL: http://creativecommons.org/licenses/by/4.0/
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