A novel antibody combination to identify KIR2DS2high natural killer cells in KIR2DL3/L2/S2 heterozygous donors.
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Authors
Rettman, Pauline
Bastidas-Legarda, Leidy Y
Fulton, Rebecca
Capizzuto, Valentina
Naiyer, Mumtaz
Publication Date
2019-01Journal Title
HLA
ISSN
2059-2302
Publisher
Wiley
Volume
93
Issue
1
Pages
32-35
Language
eng
Type
Article
This Version
AM
Physical Medium
Print
Metadata
Show full item recordCitation
Blunt, M. D., Rettman, P., Bastidas-Legarda, L. Y., Fulton, R., Capizzuto, V., Naiyer, M., Traherne, J., & et al. (2019). A novel antibody combination to identify KIR2DS2high natural killer cells in KIR2DL3/L2/S2 heterozygous donors.. HLA, 93 (1), 32-35. https://doi.org/10.1111/tan.13413
Abstract
The killer cell immunoglobulin-like receptor (KIR) KIR2DS2 induces natural killer (NK) cell activation upon ligation and in genetic studies is associated with protection against certain cancers and viral infections. One of the difficulties in understanding KIR2DS2 has been that ligands have been hard to define. In part, this is because the high sequence homology between KIR2DS2 and KIR2DL3/KIR2DL2 has made it difficult to make antibodies that specifically detect NK cells expressing KIR2DS2. Using transfected NK cell line (NKL) cells and primary human samples, we report the identification of a novel antibody combination which allows identification of NK cells with relatively high expression of KIR2DS2. This separation is sufficient to examine primary human NK cell activation in response to KIR2DS2 specific ligands.
Keywords
Killer Cells, Natural, Cells, Cultured, Humans, Virus Diseases, Neoplasms, Antibodies, Flow Cytometry, Cell Separation, Immunophenotyping, Lymphocyte Activation, Immunologic Surveillance, Heterozygote, Receptors, KIR, Receptors, KIR2DL2, Receptors, KIR2DL3
Sponsorship
European Research Council (695551)
Identifiers
External DOI: https://doi.org/10.1111/tan.13413
This record's URL: https://www.repository.cam.ac.uk/handle/1810/286657
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