S1PR1 drives a feedforward signalling loop to regulate BATF3 and the transcriptional programme of Hodgkin lymphoma cells.
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Authors
Vrzalikova, K
Ibrahim, M
Vockerodt, M
Perry, T
Margielewska, S
Lupino, L
Nagy, E
Soilleux, E
Liebelt, D
Hollows, R
Last, A
Reynolds, G
Abdullah, M
Curley, H
Care, M
Krappmann, D
Tooze, R
Allegood, J
Spiegel, S
Wei, W
Woodman, CBJ
Murray, PG
Publication Date
2018-01Journal Title
Leukemia
ISSN
0887-6924
Publisher
Springer Science and Business Media LLC
Volume
32
Issue
1
Pages
214-223
Language
eng
Type
Article
This Version
VoR
Physical Medium
Print-Electronic
Metadata
Show full item recordCitation
Vrzalikova, K., Ibrahim, M., Vockerodt, M., Perry, T., Margielewska, S., Lupino, L., Nagy, E., et al. (2018). S1PR1 drives a feedforward signalling loop to regulate BATF3 and the transcriptional programme of Hodgkin lymphoma cells.. Leukemia, 32 (1), 214-223. https://doi.org/10.1038/leu.2017.275
Abstract
The Hodgkin/Reed-Sternberg cells of classical Hodgkin lymphoma (HL) are characterised by the aberrant activation of multiple signalling pathways. Here we show that a subset of HL displays altered expression of sphingosine-1-phosphate (S1P) receptors (S1PR)s. S1P activates phosphatidylinositide 3-kinase (PI3-K) in these cells that is mediated by the increased expression of S1PR1 and the decreased expression of S1PR2. We also showed that genes regulated by the PI3-K signalling pathway in HL cell lines significantly overlap with the transcriptional programme of primary HRS cells. Genes upregulated by the PI3-K pathway included the basic leucine zipper transcription factor, ATF-like 3 (BATF3), which is normally associated with the development of dendritic cells. Immunohistochemistry confirmed that BATF3 was expressed in HRS cells of most HL cases. In contrast, in normal lymphoid tissues, BATF3 expression was confined to a small fraction of CD30-positive immunoblasts. Knockdown of BATF3 in HL cell lines revealed that BATF3 contributed to the transcriptional programme of primary HRS cells, including the upregulation of S1PR1. Our data suggest that disruption of this potentially oncogenic feedforward S1P signalling loop could provide novel therapeutic opportunities for patients with HL.
Keywords
Basic-Leucine Zipper Transcription Factors, Cell Line, Cell Line, Tumor, Gene Expression Regulation, Neoplastic, HEK293 Cells, Hodgkin Disease, Humans, Phosphatidylinositol 3-Kinases, Receptors, Lysosphingolipid, Signal Transduction, Sphingosine-1-Phosphate Receptors, Transcription, Genetic, Tumor Cells, Cultured
Identifiers
External DOI: https://doi.org/10.1038/leu.2017.275
This record's URL: https://www.repository.cam.ac.uk/handle/1810/287150
Rights
Attribution-NonCommercial-NoDerivatives 4.0 International
Licence URL: https://creativecommons.org/licenses/by-nc-nd/4.0/
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