GAL4 Drivers Specific for Type Ib and Type Is Motor Neurons in Drosophila.
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Publication Date
2019-02-07Journal Title
G3 (Bethesda)
ISSN
2160-1836
Publisher
Oxford University Press (OUP)
Volume
9
Issue
2
Pages
453-462
Language
eng
Type
Article
This Version
AM
Physical Medium
Electronic
Metadata
Show full item recordCitation
Perez Moreno, J., & O'Kane, C. (2019). GAL4 Drivers Specific for Type Ib and Type Is Motor Neurons in Drosophila.. G3 (Bethesda), 9 (2), 453-462. https://doi.org/10.1534/g3.118.200809
Abstract
The Drosophila melanogaster larval neuromuscular system is extensively used by researchers to study neuronal cell biology, and Drosophila glutamatergic motor neurons have become a major model system. There are two main Types of glutamatergic motor neurons, Ib and Is, with different structural and physiological properties at synaptic level at the neuromuscular junction. To generate genetic tools to identify and manipulate motor neurons of each Type, we screened for GAL4 driver lines for this purpose. Here we describe GAL4 drivers specific for examples of neurons within each Type, Ib or Is. These drivers showed high expression levels and were expressed in only few motor neurons, making them amenable tools for specific studies of both axonal and synapse biology in identified Type I motor neurons.
Keywords
Motor Neurons, Neuromuscular Junction, Animals, Drosophila melanogaster, Glutamic Acid, Drosophila Proteins, Green Fluorescent Proteins, Transcription Factors
Relationships
Is supplemented by: https://doi.org/10.17863/CAM.33651
Sponsorship
This work was supported by grant BB/L021706/1 from the UK Biotechnology and Biological Sciences Research Council to CJO’K, and Marie Sklodowska-Curie 19 fellowship 745007 from the European Union Horizon 2020 research and innovation programme to JJPM.
Funder references
Biotechnology and Biological Sciences Research Council (BB/L021706/1)
European Commission Horizon 2020 (H2020) Marie Sk?odowska-Curie actions (745007)
Identifiers
External DOI: https://doi.org/10.1534/g3.118.200809
This record's URL: https://www.repository.cam.ac.uk/handle/1810/287337
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