The subcellular organisation of Saccharomyces cerevisiae.
Current opinion in chemical biology
MetadataShow full item record
Nightingale, D., Geladaki, A., Breckels, L. M., Oliver, S., & Lilley, K. (2019). The subcellular organisation of Saccharomyces cerevisiae.. Current opinion in chemical biology, 48 86-95. https://doi.org/10.1016/j.cbpa.2018.10.026
Subcellular protein localisation is essential for the mechanisms that govern cellular homeostasis. The ability to understand processes leading to this phenomenon will therefore enhance our understanding of cellular function. Here we review recent developments in this field with regard to mass spectrometry, fluorescence microscopy and computational prediction methods. We highlight relative strengths and limitations of current methodologies focussing particularly on studies in the yeast Saccharomyces cerevisiae. We further present the first cell-wide spatial proteome map of S. cerevisiae, generated using hyperLOPIT, a mass spectrometry-based protein correlation profiling technique. We compare protein subcellular localisation assignments from this map, with two published fluorescence microscopy studies and show that confidence in localisation assignment is attained using multiple orthogonal methods that provide complementary data.
Saccharomyces cerevisiae, Saccharomyces cerevisiae Proteins, Microscopy, Fluorescence, Proteomics, Mass Spectrometry
BBSRC and Wellcome Trust
WELLCOME TRUST (108441/Z/15/Z)
Biotechnology and Biological Sciences Research Council (BB/L002817/1)
WELLCOME TRUST (110170/Z/15/Z)
Embargo Lift Date
External DOI: https://doi.org/10.1016/j.cbpa.2018.10.026
This record's URL: https://www.repository.cam.ac.uk/handle/1810/287525
Attribution 4.0 International
Licence URL: https://creativecommons.org/licenses/by/4.0/