Isolating Chromosomes of the Komodo Dragon: New Tools for Comparative Mapping and Sequence Assembly.

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dc.contributor.authorIannucci, Alessio
dc.contributor.authorAltmanová, Marie
dc.contributor.authorCiofi, Claudio
dc.contributor.authorFerguson-Smith, Malcolm
dc.contributor.authorPereira, Jorge C
dc.contributor.authorRehák, Ivan
dc.contributor.authorStanyon, Roscoe
dc.contributor.authorVelenský, Petr
dc.contributor.authorRovatsos, Michail
dc.contributor.authorKratochvíl, Lukáš
dc.contributor.authorJohnson Pokorná, Martina
dc.date.accessioned2019-01-15T00:31:13Z
dc.date.available2019-01-15T00:31:13Z
dc.date.issued2019
dc.identifier.issn1424-8581
dc.identifier.urihttps://www.repository.cam.ac.uk/handle/1810/287990
dc.description.abstractWe developed new tools to build a high-quality chromosomal map of the Komodo dragon (Varanus komodoensis) available for cross-species phylogenomic analyses. First, we isolated chromosomes by flow sorting and determined the chromosome content of each flow karyotype peak by FISH. We then isolated additional Komodo dragon chromosomes by microdissection and amplified chromosome-specific DNA pools. The chromosome-specific DNA pools can be sequenced, assembled, and mapped by next-generation sequencing technology. The chromosome-specific paint probes can be used to investigate karyotype evolution through cross-species chromosome painting. Overall, the set of chromosome-specific DNA pools of V. komodoensis provides new tools for detailed phylogenomic analyses of Varanidae and squamates in general.
dc.description.sponsorshipnone
dc.format.mediumPrint-Electronic
dc.languageeng
dc.publisherS. Karger AG
dc.subjectChromosome flow sorting
dc.subjectLaser capture
dc.subjectMicrodissection
dc.subjectMolecular cytogenetics
dc.subjectVaranus komodoensis
dc.subjectAnimals
dc.subjectChromosome Banding
dc.subjectChromosome Mapping
dc.subjectChromosome Painting
dc.subjectChromosomes
dc.subjectDNA
dc.subjectDNA Probes
dc.subjectFemale
dc.subjectHigh-Throughput Nucleotide Sequencing
dc.subjectKaryotype
dc.subjectLizards
dc.titleIsolating Chromosomes of the Komodo Dragon: New Tools for Comparative Mapping and Sequence Assembly.
dc.typeArticle
prism.endingPage131
prism.issueIdentifier1-2
prism.publicationDate2019
prism.publicationNameCytogenet Genome Res
prism.startingPage123
prism.volume157
dc.identifier.doi10.17863/CAM.35310
rioxxterms.versionofrecord10.1159/000496171
rioxxterms.versionAM
rioxxterms.licenseref.urihttp://www.rioxx.net/licenses/all-rights-reserved
rioxxterms.licenseref.startdate2019-01-15
dc.contributor.orcidFerguson-Smith, Malcolm [0000-0001-9372-1381]
dc.identifier.eissn1424-859X
rioxxterms.typeJournal Article/Review
cam.issuedOnline2019-01-15


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