Mobilization of Pack-CACTA transposons in Arabidopsis suggests the mechanism of gene shuffling.
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Publication Date
2019-02-20Journal Title
Nucleic Acids Res
ISSN
0305-1048
Publisher
Oxford University Press (OUP)
Volume
47
Issue
3
Pages
1311-1320
Language
eng
Type
Article
This Version
VoR
Physical Medium
Print
Metadata
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Catoni, M., Jonesman, T., Cerruti, E., & Paszkowski, J. (2019). Mobilization of Pack-CACTA transposons in Arabidopsis suggests the mechanism of gene shuffling.. Nucleic Acids Res, 47 (3), 1311-1320. https://doi.org/10.1093/nar/gky1196
Abstract
Pack-TYPE transposons are a unique class of potentially mobile non-autonomous elements that can capture, merge and relocate fragments of chromosomal DNA. It has been postulated that their activity accelerates the evolution of host genes. However, this important presumption is based only on the sequences of currently inactive Pack-TYPE transposons and the acquisition of chromosomal DNA has not been recorded in real time. Analysing the DNA copy number variation in hypomethylated Arabidopsis lines, we have now for the first time witnessed the mobilization of novel Pack-TYPE elements related to the CACTA transposon family, over several plant generations. Remarkably, these elements can insert into genes as closely spaced direct repeats and they frequently undergo incomplete excisions, resulting in the deletion of one of the end sequences. These properties suggest a mechanism of efficient acquisition of genic DNA residing between neighbouring Pack-TYPE transposons and its subsequent mobilization. Our work documents crucial steps in the formation of in vivo novel Pack-TYPE transposons, and thus the possible mechanism of gene shuffling mediated by this type of mobile element.
Keywords
Chromosomes, Plant, Arabidopsis, DNA Transposable Elements, DNA Methylation, Genome, Plant, DNA Copy Number Variations
Sponsorship
European Research Council (322621)
Gatsby Charitable Foundation (unknown)
Identifiers
External DOI: https://doi.org/10.1093/nar/gky1196
This record's URL: https://www.repository.cam.ac.uk/handle/1810/288179
Rights
Attribution-NonCommercial 4.0 International
Licence URL: https://creativecommons.org/licenses/by-nc/4.0/
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