Preliminary evaluation of a salivary urea test strip method for use in dogs.
Veterinary clinical pathology
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Sanchini, L., Hare, C. H., Restif, O., & Williams, T. (2019). Preliminary evaluation of a salivary urea test strip method for use in dogs.. Veterinary clinical pathology, 48 (3), 413-418. https://doi.org/10.1111/vcp.12765
Background: Salivary urea concentrations correlate with serum urea concentrations in dogs and humans. Salivary urea concentrations can now be determined semi-quantitatively using a salivary urea test strip method that has been validated for use in humans. Objectives: Evaluation of the repeatability of a salivary urea test strip score and correlation between the salivary urea test strip score and serum urea concentration in dogs. Methods: Intra-run and inter-run variability were determined (n=10 in triplicate). Correlation between salivary urea test strip score and serum urea concentrations in dogs was assessed using Spearman’s correlation coefficient. Receiver operator curve analysis was used to evaluate diagnostic performance of the salivary urea test strip score to identify dogs with serum urea concentration >7.4 mmol/L (upper limit of laboratory reference interval). Results: Intra-run repeatability was good (28/30 concordant results) whereas inter-run repeatability was moderate (23/30 concordant results). Salivary and serum urea concentrations showed moderate positive correlation (rs= 0.63, n=33; P<0.0001). Salivary urea test strip score ≥4 was 57% sensitive and 96% specific for detecting a serum urea concentration >7.4 mmol/L. Conclusions: Uremia can be detected using salivary urea test strips in dogs. Based on our preliminary data, salivary urea test strip scores of 1 or 2 might exclude clinically relevant uremia in most cases, however it is recommended that the salivary urea test should be repeated in dogs with a test strip score of 3. Dogs with a salivary urea test strip score of >/=4 would likely require additional investigations.
Saliva, Animals, Dogs, Urea, Reagent Strips, Female, Male
External DOI: https://doi.org/10.1111/vcp.12765
This record's URL: https://www.repository.cam.ac.uk/handle/1810/289056