Tissue- and sex-specific small RNAomes reveal sex differences in response to the environment.
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Authors
Bezler, Alexandra
West, Sean M
Duplan, Arthur
Conconi, Raffaella
Schütz, Frédéric
Gönczy, Pierre
Piano, Fabio
Gunsalus, Kristin
Keller, Laurent
Publication Date
2019-02-21Journal Title
PLoS Genet
ISSN
1553-7390
Publisher
PLOS
Volume
15
Issue
2
Pages
e1007905-e1007905
Language
eng
Type
Article
This Version
VoR
Metadata
Show full item recordCitation
Bezler, A., Braukmann, F., West, S. M., Duplan, A., Conconi, R., Schütz, F., Gönczy, P., et al. (2019). Tissue- and sex-specific small RNAomes reveal sex differences in response to the environment.. PLoS Genet, 15 (2), e1007905-e1007905. https://doi.org/10.1371/journal.pgen.1007905
Abstract
RNA interference (RNAi) related pathways are essential for germline development and fertility in metazoa and can contribute to inter- and trans-generational inheritance. In the nematode Caenorhabditis elegans, environmental double-stranded RNA provided by feeding can lead to heritable changes in phenotype and gene expression. Notably, transmission efficiency differs between the male and female germline, yet the underlying mechanisms remain elusive. Here we use high-throughput sequencing of dissected gonads to quantify sex-specific endogenous piRNAs, miRNAs and siRNAs in the C. elegans germline and the somatic gonad. We identify genes with exceptionally high levels of secondary 22G RNAs that are associated with low mRNA expression, a signature compatible with silencing. We further demonstrate that contrary to the hermaphrodite germline, the male germline, but not male soma, is resistant to environmental RNAi triggers provided by feeding, in line with previous work. This sex-difference in silencing efficacy is associated with lower levels of gonadal RNAi amplification products. Moreover, this tissue- and sex-specific RNAi resistance is regulated by the germline, since mutant males with a feminized germline are RNAi sensitive. This study provides important sex- and tissue-specific expression data of miRNA, piRNA and siRNA as well as mechanistic insights into sex-differences of gene regulation in response to environmental cues.
Sponsorship
This work was funded by grants from the
Swiss National Science Foundation and an
advanced European Research Council grant to Laurent Keller,
grants from Cancer Research UK (C13474/A18583,
C6946/A14492) and the Wellcome Trust (104640/
Z/14/Z, 092096/Z/10/Z) to Eric A. Miska, and grants from
the National Institutes of Health to Sean M. West (NIGMSNHRA 5F32GM100614) and to Fabio Piano and Kristin Gunsalus
(NHGRI U01 HG004276, NICHD R01 HD046236),
and by research funding from New York University
Abu Dhabi to Fabio Piano and Kristin Gunsalus.
Funder references
Wellcome Trust (092096/Z/10/Z)
Cancer Research UK (18583)
Cancer Research UK (A14492)
WELLCOME TRUST (104640/Z/14/Z)
Identifiers
External DOI: https://doi.org/10.1371/journal.pgen.1007905
This record's URL: https://www.repository.cam.ac.uk/handle/1810/289431
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