Robust, universal biomarker assay to detect senescent cells in biological specimens.
Published version
Peer-reviewed
Repository URI
Repository DOI
Change log
Authors
Evangelou, Konstantinos
Lougiakis, Nikolaos
Rizou, Sophia V
Kotsinas, Athanassios
Kletsas, Dimitris
Abstract
Cellular senescence contributes to organismal development, aging, and diverse pathologies, yet available assays to detect senescent cells remain unsatisfactory. Here, we designed and synthesized a lipophilic, biotin-linked Sudan Black B (SBB) analogue suitable for sensitive and specific, antibody-enhanced detection of lipofuscin-containing senescent cells in any biological material. This new hybrid histo-/immunochemical method is easy to perform, reliable, and universally applicable to assess senescence in biomedicine, from cancer research to gerontology.
Description
Keywords
Sudan Black B, aging, biotin‐linked compounds, immunohistochemistry, senescence, Animals, Biological Assay, Biomarkers, Biotin, Cell Line, Tumor, Cellular Senescence, Humans, Mice
Journal Title
Aging Cell
Conference Name
Journal ISSN
1474-9718
1474-9726
1474-9726
Volume Title
16
Publisher
Wiley