Norbornene Probes for the Detection of Cysteine Sulfenic Acid in Cells.
Accepted version
Peer-reviewed
Repository URI
Repository DOI
Change log
Authors
Alcock, Lisa J https://orcid.org/0000-0001-9068-0756
Oliveira, Bruno L
Deery, Michael J
Pukala, Tara L https://orcid.org/0000-0001-7391-1436
Perkins, Michael V
Abstract
Norbornene derivatives were validated as probes for cysteine sulfenic acid on proteins and in live cells. Trapping sulfenic acids with norbornene probes is highly selective and revealed a different reactivity profile than the traditional dimedone reagent. The norbornene probe also revealed a superior chemoselectivity when compared to a commonly used dimedone probe. Together, these results advance the study of cysteine oxidation in biological systems.
Description
Keywords
Cysteine, Molecular Probes, Norbornanes, Oxidation-Reduction, Sulfenic Acids
Journal Title
ACS Chem Biol
Conference Name
Journal ISSN
1554-8929
1554-8937
1554-8937
Volume Title
14
Publisher
American Chemical Society (ACS)
Publisher DOI
Rights
All rights reserved
Sponsorship
Engineering and Physical Sciences Research Council (EP/M003647/1)
The Royal Society (uf110046)
European Research Council (676832)
Royal Society (URF\R\180019)
The Royal Society (uf110046)
European Research Council (676832)
Royal Society (URF\R\180019)