Centriolar satellites are acentriolar assemblies of centrosomal proteins
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Gergely, F. (2019). Centriolar satellites are acentriolar assemblies of centrosomal proteins. EMBO Journal, 38 (14. e101082)https://doi.org/10.15252/embj.2018101082
Centrioles are core structural elements of both centrosomes and cilia. Although cytoplasmic granules called centriolar satellites have been observed around these structures, lack of a comprehensive inventory of satellite proteins impedes our understanding of their ancestry. To address this, we performed mass spectrometry (MS)-based proteome profiling of centriolar satellites obtained by affinity purification of their key constituent, PCM1, from sucrose gradient fractions. We defined an interactome consisting of 223 proteins, which showed striking enrichment in centrosome components. The proteome also contained new structural and regulatory factors with roles in ciliogenesis. Quantitative MS on whole-cell and centriolar satellite proteomes of acentriolar cells was performed to reveal dependencies of satellite composition on intact centrosomes. Although, most components remained associated with PCM1 in acentriolar cells, reduced cytoplasmic and satellite levels were observed for a subset of centrosomal proteins. These results demonstrate that centriolar satellites and centrosomes form independently but share a substantial fraction of their proteomes. Dynamic exchange of proteins between these organelles could facilitate their adaptation to changing cellular environments during development, stress response and tissue homeostasis.
This work was funded by Cancer Research UK (C14303/A17197 to M.M and C14303/A17043 to F.G). J.T was supported by a Deutsche Forschungsgemeinschaft research fellowship, HA‐8069/1‐1. We acknowledge support from NIHR Cambridge Biomedical Research Centre, the University of Cambridge and Hutchison Whampoa Ltd.
Cancer Research UK (CB4180)
Cancer Research UK (C14303/A17197)
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External DOI: https://doi.org/10.15252/embj.2018101082
This record's URL: https://www.repository.cam.ac.uk/handle/1810/292768
Attribution 4.0 International
Licence URL: https://creativecommons.org/licenses/by/4.0/