A cell-engineered system to assess tumor cell sensitivity to CD8+ T cell-mediated cytotoxicity
Taylor & Francis
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de la Roche, M. (2019). A cell-engineered system to assess tumor cell sensitivity to CD8+ T cell-mediated cytotoxicity. OncoImmunology, 8 (8. e1599635)https://doi.org/10.1080/2162402X.2019.1599635
In vitro assays that evaluate CD8+ T cell-mediated cytotoxicity are important to aid in the development of novel therapeutic approaches to enhance anti-tumor immune responses. Here, we describe a novel cytotoxicity co-culture assay that circumvents the problem of highly variable allogeneic responses and obviates the constraints of HLA-restriction between effector and target cells. We show that this assay can be easily applied to a panel of tumor cell lines to provide additional insights into intrinsic drivers of sensitivity/resistance to T cell-mediated killing, and to evaluate the impact of targeted therapies on both tumor and T cell compartments.
Maike de la Roche is supported by a Sir Henry Dale Fellowship jointly funded by the Wellcome Trust and the Royal Society (WT107609) and Cancer Research UK (A22257).
WELLCOME TRUST (107609/Z/15/Z)
Cancer Research UK (C14303_do not transfer)
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External DOI: https://doi.org/10.1080/2162402X.2019.1599635
This record's URL: https://www.repository.cam.ac.uk/handle/1810/293689
Attribution-NonCommercial-NoDerivatives 4.0 International
Licence URL: http://creativecommons.org/licenses/by-nc-nd/4.0/