A MILI-independent piRNA biogenesis pathway empowers partial germline reprogramming.
Authors
Berrens, Rebecca V
Carrieri, Claudia
Reik, Wolf
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Abstract
In mice, the pathway involving PIWI and PIWI-interacting RNA (PIWI-piRNA) is essential to re-establish transposon silencing during male-germline reprogramming. The cytoplasmic PIWI protein MILI mediates piRNA-guided transposon RNA cleavage as well as piRNA amplification. MIWI2's binding to piRNA and its nuclear localization are proposed to be dependent upon MILI function. Here, we demonstrate the existence of a piRNA biogenesis pathway that sustains partial MIWI2 function and reprogramming activity in the absence of MILI.
Publication Date
2017-07
Online Publication Date
2017-05-22
Acceptance Date
2017-04-26
Keywords
Animals, Argonaute Proteins, Gene Expression Regulation, Mice, Mice, Knockout, RNA, Small Interfering
Journal Title
Nat Struct Mol Biol
Journal ISSN
1545-9993
1545-9985
1545-9985
Volume Title
24
Publisher
Springer Science and Business Media LLC
Rights
All rights reserved