Comment on "Protein assemblies ejected directly from native membranes yield complexes for mass spectrometry".
Science (New York, N.Y.)
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Hirst, J., Kunji, E., & Walker, J. (2019). Comment on "Protein assemblies ejected directly from native membranes yield complexes for mass spectrometry".. Science (New York, N.Y.), 366 (6466)https://doi.org/10.1126/science.aaw9830
Chorev et al. (1) describe mass spectrometry on mitochondrial membrane proteins ionized directly from their native environment. However, the assignments made to measured masses are incorrect or inconclusive, and lack experimental validation. The proteins are not in their ‘native’ condition: they have been stripped of tightly-bound lipids, and the complexes are fragmented or in physiologically irrelevant oligomeric states.
Lipids, Membrane Proteins, Mass Spectrometry
External DOI: https://doi.org/10.1126/science.aaw9830
This record's URL: https://www.repository.cam.ac.uk/handle/1810/300183
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