Comment on "Protein assemblies ejected directly from native membranes yield complexes for mass spectrometry".
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Publication Date
2019-11-07Journal Title
Science (New York, N.Y.)
ISSN
0036-8075
Volume
366
Issue
6466
Language
eng
Type
Article
This Version
AM
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Physical Medium
Print-Electronic
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Hirst, J., Kunji, E., & Walker, J. (2019). Comment on "Protein assemblies ejected directly from native membranes yield complexes for mass spectrometry".. Science (New York, N.Y.), 366 (6466)https://doi.org/10.1126/science.aaw9830
Abstract
Chorev et al. (1) describe mass spectrometry on mitochondrial membrane proteins ionized directly from their native environment. However, the assignments made to measured masses are incorrect or inconclusive, and lack experimental validation. The proteins are not in their ‘native’ condition: they have been stripped of tightly-bound lipids, and the complexes are fragmented or in physiologically irrelevant oligomeric states.
Keywords
Lipids, Membrane Proteins, Mass Spectrometry
Sponsorship
MRC (MC_UU_00015/1)
MRC (MC_EX_MR/M009858/1)
MRC (MC_U105663139)
MRC (MC_UU_00015/2)
MRC (MC_U105663141)
MRC (MC_U105663150)
MRC (MC_UU_00015/8)
MRC (MC_UU_00015/2)
MRC (MC_UU_00015/8)
Identifiers
External DOI: https://doi.org/10.1126/science.aaw9830
This record's URL: https://www.repository.cam.ac.uk/handle/1810/300183
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