Microinjection of superior cervical ganglion neurons for studying axon degeneration
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Peer-reviewed
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Abstract
Primary cultures of neurons of the peripheral nervous system have been successfully used for studying many aspects of neuronal development and survival, including investigations into the mechanisms of axon degeneration. In this chapter we describe how to prepare and microinject dissociated cultures of sympathetic neurons of the superior cervical ganglion (SCG) specifically for use in highly controlled and targeted assays of axon survival and degeneration.
Description
Keywords
Axon degeneration, Axon survival, Axon transection, Dissociated SCG culture, Microinjection, Superior cervical ganglion, Wallerian degeneration, Animals, Aphidicolin, Axons, Axotomy, Fluorescent Dyes, Mice, Microinjections, Microscopy, Fluorescence, Microscopy, Phase-Contrast, Nerve Growth Factor, Primary Cell Culture, Sensory Receptor Cells, Solutions, Superior Cervical Ganglion
Journal Title
Methods in Molecular Biology
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Journal ISSN
1064-3745
1940-6029
1940-6029
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Publisher
Springer Nature
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Except where otherwised noted, this item's license is described as Attribution 4.0 International (CC BY)
Sponsorship
Medical Research Council (MR/N004582/1)
Wellcome Trust (210904/Z/18/Z)
Biotechnology and Biological Sciences Research Council (BB/S009582/1)
Parkinson's UK (G-1602)
Medical Research Council (G1000702/1)
Wellcome Trust (210904/Z/18/Z)
Biotechnology and Biological Sciences Research Council (BB/S009582/1)
Parkinson's UK (G-1602)
Medical Research Council (G1000702/1)