Quantitative phase and polarization imaging through an optical fiber applied to detection of early esophageal tumorigenesis.
Journal of biomedical optics
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Gordon, G., Joseph, J., Alcolea Delgado, M., Sawyer, T., Williams, C., Fitzpatrick, C. R. M., Jones, P., et al. (2019). Quantitative phase and polarization imaging through an optical fiber applied to detection of early esophageal tumorigenesis.. Journal of biomedical optics, 24 (12), 1-13. https://doi.org/10.1117/1.jbo.24.12.126004
Phase and polarisation of coherent light are highly perturbed by interaction with microstructural changes in pre-malignant tissue, holding promise for label-free detection of early tumours in endoscopically accessible tissues such as the gastrointestinal tract. Flexible optical multi-core fibre bundles (MCFs) used in conventional diagnostic endoscopy and endomicroscopy scramble phase and polarisation, restricting clinicians instead to low-contrast amplitude-only imaging. Here, we apply a transmission matrix characterisation approach to produce full-field en-face images of amplitude, quantitative phase and resolved polarimetric properties through an MCF. We first demonstrate imaging and quantification of biologically-relevant amounts of optical scattering and birefringence in tissue-mimicking phantoms. We present an entropy metric that enables imaging of phase heterogeneity, indicative of disordered tissue microstructure associated with early tumours. Finally, we demonstrate that the spatial distribution of phase and polarisation information enables label-free visualisation of early tumours in oesophageal mouse tissue that are not identifiable using conventional amplitude-only information.
This work was funded by Cancer Research UK (C47594/A16267, C14303/A17197, C47594/A21102); the European Union Seventh Framework Agreement (FP7-PEOPLE-2013-CIG-630729); and pump priming awards from the Cancer Research UK Cambridge Centre, including dedicated funding from the Early Detection Programme (A20976).
European Commission (630729)
Cancer Research UK (16267)
Cancer Research UK (C14303/A17197)
Cancer Research UK (21102)
Cancer Research UK (24669)
Cancer Research UK (C609/A17257)
Cancer Research UK (C20/A20976)
Cancer Research UK (A25117)
External DOI: https://doi.org/10.1117/1.jbo.24.12.126004
This record's URL: https://www.repository.cam.ac.uk/handle/1810/300863
Attribution 4.0 International (CC BY)
Licence URL: http://creativecommons.org/licenses/by/4.0/