A high infectious simian adenovirus type 23 vector based vaccine efficiently protects common marmosets against Zika virus infection.
Authors
Luo, Shengxue
Zhao, Wei
Ma, Xiaorui
Zhang, Panli
Liu, Bochao
Zhang, Ling
Wang, Wenjing
Wang, Yuanzhan
Fu, Yongshui
Allain, Jean-Pierre
Li, Tingting
Publication Date
2020-02Journal Title
PLoS Negl Trop Dis
ISSN
1935-2727
Publisher
Public Library of Science (PLoS)
Volume
14
Issue
2
Language
en
Type
Article
This Version
VoR
Metadata
Show full item recordCitation
Luo, S., Zhao, W., Ma, X., Zhang, P., Liu, B., Zhang, L., Wang, W., et al. (2020). A high infectious simian adenovirus type 23 vector based vaccine efficiently protects common marmosets against Zika virus infection.. PLoS Negl Trop Dis, 14 (2) https://doi.org/10.1371/journal.pntd.0008027
Abstract
Zika virus (ZIKV) has spread in many countries or territories causing severe neurologic complications with potential fatal outcomes. The small primate common marmosets are susceptible to ZIKV, mimicking key features of human infection. Here, a novel simian adenovirus type 23 vector-based vaccine expressing ZIKV pre-membrane-envelope proteins (Sad23L-prM-E) was produced in high infectious titer. Due to determination of immunogenicity in mice, a single-dose of 3×108 PFU Sad23L-prM-E vaccine was intramuscularly inoculated to marmosets. This vaccine raised antibody titers of 104.07 E-specific and 103.13 neutralizing antibody (NAb), as well as robust specific IFN-γ secreting T-cell response (1,219 SFCs/106 cells) to E peptides. The vaccinated marmosets, upon challenge with a high dose of ZIKV (105 PFU) six weeks post prime immunization, reduced viremia by more than 100 folds, and the low level of detectable viral RNA (<103 copies/ml) in blood, saliva, urine and feces was promptly eliminated when the secondary NAb (titer >103.66) and T-cell response (>726 SFCs/106 PBMCs) were acquired 1-2 weeks post exposure to ZIKV, while non-vaccinated control marmosets developed long-term high titer of ZIKV (105.73 copies/ml) (P<0.05). No significant pathological lesions were observed in marmoset tissues. Sad23L-prM-E vaccine was detectable in spleen, liver and PBMCs at least 4 months post challenge. In conclusion, a prime immunization with Sad23L-prM-E vaccine was able to protect marmosets against ZIKV infection when exposed to a high dose of ZIKV. This Sad23L-prM-E vaccine is a promising vaccine candidate for prevention of ZIKV infection in humans.
Keywords
Research Article, Research and analysis methods, Biology and life sciences, Medicine and health sciences
Sponsorship
National Natural Science Foundation of China (31770185, 31970886 and 31500134)
National Natural Science Foundation of China (81871655)
Guangzhou major project of industry-university-research cooperation and collaborative innovation (201704020083)
Innovative R&D Team Introduction Program of Guangdong (2014ZT05S123)
Guangdong Province Universities and Colleges Pearl River Scholar Funded Scheme (2017)
the National Key Research and Development Program (2017YFD0500305)
Identifiers
pntd-d-19-01220
External DOI: https://doi.org/10.1371/journal.pntd.0008027
This record's URL: https://www.repository.cam.ac.uk/handle/1810/302039
Rights
Attribution 4.0 International (CC BY 4.0)
Licence URL: https://creativecommons.org/licenses/by/4.0/
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