High-throughput, multi-parametric, and correlative fluorescence lifetime imaging
Publication Date
2020-02-19Journal Title
Methods and Applications in Fluorescence
Publisher
IOP Publishing
Volume
8
Issue
2
Language
en
Type
Article
This Version
VoR
Metadata
Show full item recordCitation
Poudel, C., Mela, I., & Kaminski, C. F. (2020). High-throughput, multi-parametric, and correlative fluorescence lifetime imaging. Methods and Applications in Fluorescence, 8 (2) https://doi.org/10.1088/2050-6120/ab7364
Description
Funder: Infinitus (China), Ltd.
Funder: MedImmune (AstraZeneca); doi: https://doi.org/10.13039/501100004628
Abstract
Abstract: In this review, we discuss methods and advancements in fluorescence lifetime imaging microscopy that permit measurements to be performed at faster speed and higher resolution than previously possible. We review fast single-photon timing technologies and the use of parallelized detection schemes to enable high-throughput and high content imaging applications. We appraise different technological implementations of fluorescence lifetime imaging, primarily in the time-domain. We also review combinations of fluorescence lifetime with other imaging modalities to capture multi-dimensional and correlative information from a single sample. Throughout the review, we focus on applications in biomedical research. We conclude with a critical outlook on current challenges and future opportunities in this rapidly developing field.
Keywords
Paper, Special Issue on Fluorescence Lifetime Imaging (FLIM): from Fundamentals to Applications, fluorescence lifetime, acquisition time, SPAD array, high throughput, FLIM, multi-parametric imaging, correlative FLIM
Sponsorship
H2020 Marie Sklodowska-Curie Actions (722380 (SUPUVIR), H2020-MSCA-ITN-2016)
Wellcome Trust (089703/Z/ 09/Z, 3- 3249/Z/16/Z)
Engineering and Physical Sciences Research Council (EP/ H018301/1, EP/L015889/1)
Medical Research Council (MR/K015850/1, MR/K02292X/1)
Identifiers
mafab7364, ab7364, maf-100664.r2
External DOI: https://doi.org/10.1088/2050-6120/ab7364
This record's URL: https://www.repository.cam.ac.uk/handle/1810/302437
Rights
Attribution 4.0 International (CC BY 4.0)
Licence URL: https://creativecommons.org/licenses/by/4.0/
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