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dc.contributor.authorPoudel, Chetan
dc.contributor.authorMela, Ioanna
dc.contributor.authorKaminski, Clemens F
dc.date.accessioned2020-02-20T14:32:33Z
dc.date.available2020-02-20T14:32:33Z
dc.date.issued2020-02-19
dc.date.submitted2019-08-13
dc.identifier.othermafab7364
dc.identifier.otherab7364
dc.identifier.othermaf-100664.r2
dc.identifier.urihttps://www.repository.cam.ac.uk/handle/1810/302437
dc.descriptionFunder: Infinitus (China), Ltd.
dc.descriptionFunder: MedImmune (AstraZeneca); doi: https://doi.org/10.13039/501100004628
dc.description.abstractAbstract: In this review, we discuss methods and advancements in fluorescence lifetime imaging microscopy that permit measurements to be performed at faster speed and higher resolution than previously possible. We review fast single-photon timing technologies and the use of parallelized detection schemes to enable high-throughput and high content imaging applications. We appraise different technological implementations of fluorescence lifetime imaging, primarily in the time-domain. We also review combinations of fluorescence lifetime with other imaging modalities to capture multi-dimensional and correlative information from a single sample. Throughout the review, we focus on applications in biomedical research. We conclude with a critical outlook on current challenges and future opportunities in this rapidly developing field.
dc.languageen
dc.publisherIOP Publishing
dc.rightsAttribution 4.0 International (CC BY 4.0)en
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/en
dc.subjectPaper
dc.subjectSpecial Issue on Fluorescence Lifetime Imaging (FLIM): from Fundamentals to Applications
dc.subjectfluorescence lifetime
dc.subjectacquisition time
dc.subjectSPAD array
dc.subjecthigh throughput
dc.subjectFLIM
dc.subjectmulti-parametric imaging
dc.subjectcorrelative FLIM
dc.titleHigh-throughput, multi-parametric, and correlative fluorescence lifetime imaging
dc.typeArticle
dc.date.updated2020-02-20T14:32:33Z
prism.issueIdentifier2
prism.publicationNameMethods and Applications in Fluorescence
prism.volume8
dc.identifier.doi10.17863/CAM.49508
dcterms.dateAccepted2020-02-06
rioxxterms.versionofrecord10.1088/2050-6120/ab7364
rioxxterms.versionVoR
rioxxterms.licenseref.urihttp://creativecommons.org/licenses/by/4.0/
dc.contributor.orcidPoudel, Chetan [0000-0002-8512-9238]
dc.contributor.orcidMela, Ioanna [0000-0002-2914-9971]
dc.contributor.orcidKaminski, Clemens F [0000-0002-5194-0962]
dc.identifier.eissn2050-6120
pubs.funder-project-idH2020 Marie Sklodowska-Curie Actions (722380 (SUPUVIR), H2020-MSCA-ITN-2016)
pubs.funder-project-idWellcome Trust (089703/Z/ 09/Z, 3- 3249/Z/16/Z)
pubs.funder-project-idEngineering and Physical Sciences Research Council (EP/ H018301/1, EP/L015889/1)
pubs.funder-project-idMedical Research Council (MR/K015850/1, MR/K02292X/1)


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Attribution 4.0 International (CC BY 4.0)
Except where otherwise noted, this item's licence is described as Attribution 4.0 International (CC BY 4.0)