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dc.contributor.authorPopa, Stephanie
dc.contributor.authorVilleneuve, Julien
dc.contributor.authorStewart, Sarah
dc.contributor.authorPerez Garcia, Esther
dc.contributor.authorPetrunkina Harrison, Anna
dc.contributor.authorMoreau, Kevin
dc.date.accessioned2020-03-10T09:36:23Z
dc.date.available2020-03-10T09:36:23Z
dc.date.issued2019-08-09
dc.identifier.issn2398-502X
dc.identifier.otherPMC6979480
dc.identifier.other32030357
dc.identifier.urihttps://www.repository.cam.ac.uk/handle/1810/303254
dc.description.abstractBackground: The fundamental process of protein secretion from eukaryotic cells has been well described for many years, yet gaps in our understanding of how this process is regulated remain. Methods: With the aim of identifying novel genes involved in the secretion of glycoproteins, we used a screening pipeline consisting of a pooled genome-wide CRISPR screen, followed by secondary siRNA screening of the hits to identify and validate several novel regulators of protein secretion. Results: We present approximately 50 novel genes not previously associated with protein secretion, many of which also had an effect on the structure of the Golgi apparatus. We further studied a small selection of hits to investigate their subcellular localisation. One of these, GPR161, is a novel Golgi-resident protein that we propose maintains Golgi structure via an interaction with golgin A5. Conclusions: This study has identified new factors for protein secretion involved in Golgi homeostasis.
dc.languageeng
dc.rightsAttribution 4.0 International
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/
dc.sourceessn: 2398-502X
dc.sourcenlmid: 101696457
dc.subjectSecretion
dc.subjectEndoplasmic reticulum
dc.subjectGolgi
dc.subjectCrispr Screen
dc.titleGenome-wide CRISPR screening identifies new regulators of glycoprotein secretion.
dc.typeArticle
dc.date.updated2020-03-10T09:36:23Z
prism.publicationNameWellcome open research
prism.volume4
dc.identifier.doi10.17863/CAM.50333
rioxxterms.versionofrecord10.12688/wellcomeopenres.15232.2
rioxxterms.versionVoR
rioxxterms.licenseref.urihttps://creativecommons.org/licenses/by/4.0/
dc.contributor.orcidPopa, Stephanie [0000-0002-3424-4674]
dc.contributor.orcidStewart, Sarah [0000-0003-3712-9898]
pubs.funder-project-idMedical Research Council (MC_UU_12012/5)
pubs.funder-project-idWellcome Trust (100574, 109152)


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Attribution 4.0 International
Except where otherwise noted, this item's licence is described as Attribution 4.0 International