Comparative Transcriptomics Reveals Clues for Differences in Pathogenicity between Hysterothylacium aduncum, Anisakis simplex sensu stricto and Anisakis pegreffii
Authors
Cavallero, Serena
Salvemini, Marco
D’Amelio, Stefano
Publication Date
2020-03-18Journal Title
Genes
Publisher
MDPI
Volume
11
Issue
3
Language
en
Type
Article
This Version
VoR
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Cavallero, S., Lombardo, F., Salvemini, M., Pizzarelli, A., Cantacessi, C., & D’Amelio, S. (2020). Comparative Transcriptomics Reveals Clues for Differences in Pathogenicity between Hysterothylacium aduncum, Anisakis simplex sensu stricto and Anisakis pegreffii. Genes, 11 (3)https://doi.org/10.3390/genes11030321
Abstract
Ascaridoid nematodes are widespread in marine fishes. Despite their major socioeconomic importance, mechanisms associated to the fish-borne zoonotic disease anisakiasis are still obscure. RNA-Seq and de-novo assembly were herein applied to RNA extracted from larvae and dissected pharynx of Hysterothylacium aduncum (HA), a non-pathogenic nematode. Assembled transcripts in HA were annotated and compared to the transcriptomes of the zoonotic species Anisakis simplex sensu stricto (AS) and Anisakis pegreffii (AP). Approximately 60,000,000 single-end reads were generated for HA, AS and AP. Transcripts in HA encoded for 30,254 putative peptides while AS and AP encoded for 20,574 and 20,840 putative peptides, respectively. Differential gene expression analyses yielded 471, 612 and 526 transcripts up regulated in the pharynx of HA, AS and AP. The transcriptomes of larvae and pharynx of HA were enriched in transcripts encoding collagen, peptidases, ribosomal proteins and in heat-shock motifs. Transcripts encoding proteolytic enzymes, anesthetics, inhibitors of primary hemostasis and virulence factors, anticoagulants and immunomodulatory peptides were up-regulated in AS and AP pharynx. This study represents the first transcriptomic characterization of a marine parasitic nematode commonly recovered in fish and probably of negligible concern for public health.
Keywords
Hysterothylacium, Anisakis, transcriptome, pharyngeal transcripts, DEGs
Sponsorship
European Society of Clinical Microbiology and Infectious Diseases (ESCMID GRANT 2015)
Identifiers
External DOI: https://doi.org/10.3390/genes11030321
This record's URL: https://www.repository.cam.ac.uk/handle/1810/303714
Rights
Licence:
https://creativecommons.org/licenses/by/4.0/