Bioinspired genotype-phenotype linkages: mimicking cellular compartmentalization for the engineering of functional proteins.
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Peer-reviewed
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Abstract
The idea of compartmentalization of genotype and phenotype in cells is key for enabling Darwinian evolution. This contribution describes bioinspired systems that use in vitro compartments-water-in-oil droplets and gel-shell beads-for the directed evolution of functional proteins. Technologies based on these principles promise to provide easier access to protein-based therapeutics, reagents for processes involving enzyme catalysis, parts for synthetic biology and materials with biological components.
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Keywords
antibodies, bioinspiration, cell compartmentalization, emulsion droplets, microfluidics, protein engineering, synthetic biology
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Interface Focus
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Journal ISSN
2042-8898
2042-8901
2042-8901
Volume Title
5
Publisher
The Royal Society
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Sponsorship
Engineering and Physical Sciences Research Council (EP/H046593/1)