Lesson of the month: novel method to quantify neutrophil uptake in early lung cancer using SPECT-CT.
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Peer-reviewed
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Abstract
Neutrophils play an important role in the lung tumour microenvironment. We hypothesised that radiolabelled neutrophils coupled to single-photon emission CT (SPECT) may non-invasively quantify neutrophil uptake in tumours from patients with non-small cell lung cancer. We demonstrated increased uptake of radiolabelled neutrophils from the blood into tumours compared with non-specific uptake using radiolabelled transferrin. Moreover, indium-111-neutrophil activity in the tumour biopsies also correlated with myeloperoxidase (MPO)-positive neutrophils. Our data support the utility of imaging with In-111-labelled neutrophils and SPECT-CT to quantify neutrophil uptake in lung cancer.
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Keywords
imaging/CT MRI etc, lung cancer, neutrophil biology, Adult, Biopsy, Female, Humans, Indium Radioisotopes, Lung Neoplasms, Male, Neoplasm Staging, Neutrophils, Tomography, Emission-Computed, Single-Photon
Journal Title
Thorax
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Journal ISSN
0040-6376
1468-3296
1468-3296
Volume Title
75
Publisher
BMJ
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All rights reserved
Sponsorship
Medical Research Council (MR/J00345X/1)
Cambridge University Hospitals NHS Foundation Trust (CUH) (146281)
Cambridge University Hospitals NHS Foundation Trust (CUH) (146281)
This research was supported by funding from the Medical Research Council
[MR/J00345X/1], the Wellcome Trust [098351/Z/12/Z], and the National Institute of Health Research (NIHR) Cambridge Biomedical Research Centre.