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Fast volumetric fluorescence imaging with multimode fibers.

Accepted version
Peer-reviewed

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Type

Article

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Authors

Wen, Zhong 
Wang, Liqiang 
Zhang, Xuanhao 
Ma, Yaoguang 
Liu, Xu 

Abstract

In this Letter, we propose a compact multimode fiber endoscope which employs wavefront shaping with a digital micromirror device (DMD). An automated single calibration step allows us to correct for optical misalignment, and the method achieves accurate focusing at various depths in the sample through rapid switching of holographic patterns by the DMD. The speed of calibration is one or two orders of magnitude faster than existing methods. The method, single calibration multimode fiber imaging (SCMFI), is compared with existing methods, and its performance is validated. We show a near diffraction limited focusing capability at imaging depths up to 110 µm with near constant lateral resolutions of 1.4 µm. Finally, we demonstrate the method for the imaging of small fluorescent beads embedded in a 3D matrix. The results indicate excellent power penetration and focusing performance. Combined with the high speed of SCMFI, this paves the way for volumetric tissue endoscopy at depth.

Description

Keywords

Calibration, Optical Fibers, Optical Imaging, Time Factors

Journal Title

Opt Lett

Conference Name

Journal ISSN

0146-9592
1539-4794

Volume Title

45

Publisher

Optica Publishing Group

Rights

All rights reserved
Sponsorship
Engineering and Physical Sciences Research Council (EP/H018301/1)
Wellcome Trust (093831/B/10/Z)
Wellcome Trust (089703/Z/09/Z)
Medical Research Council (MR/K015850/1)
Medical Research Council (MR/K02292X/1)
Engineering and Physical Sciences Research Council (EP/L015889/1)
European Commission Horizon 2020 (H2020) Marie Sk?odowska-Curie actions (722380)