Abnormal placental CD8<sup>+</sup> T-cell infiltration is a feature of fetal growth restriction and pre-eclampsia.
van der Linden, Margaretha W
Jessop, Flora A
The Journal of physiology
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Lager, S., Sovio, U., Eddershaw, E., van der Linden, M. W., Yazar, C., Cook, E., Happerfield, L., et al. (2020). Abnormal placental CD8<sup>+</sup> T-cell infiltration is a feature of fetal growth restriction and pre-eclampsia.. The Journal of physiology, 598 (23), 5555-5571. https://doi.org/10.1113/jp279532
Abstract Fetal growth restriction (FGR) and pre-eclampsia are severe, adverse pregnancy outcomes. Alterations in placental histology are frequently reported in these pregnancy complications and are often based upon scoring by pathologists. However, many alterations are also observed in placenta from uncomplicated pregnancies. Moreover, knowledge of disease state may bias assessment. We sought to perform an objective comparison of placental microscopic appearance in normal and complicated pregnancies. Placental villous tissue (n=823) and edge biopsies (n=488) from 871 individual, singleton pregnancies were collected after delivery. Cases of small-for-gestational-age (SGA) or pre-eclampsia were matched with healthy controls. A subset of the SGA cases displayed signs of FGR. Cases of pre-term delivery were also included. Tissue sections were stained with H&E or antibodies for CD8, CD14, CD31, CD79α, elastase. Images were scored by two experienced pathologists for pathological features or analysed by image analysis and stereology. Analyses were performed blind to case-control status and gestational age. Volume fraction of T-cells increased in placentas from pregnancies complicated by pre-eclampsia (adjusted odds ratio (aOR) 1.46, 95% CI 1.12-1.90) and FGR (aOR 1.64, 95% CI 1.11-2.43), whereas B-cells only increased in FGR (aOR 1.65, 95% CI 1.05-2.60). Pathological abnormalities in villous tissue were reported in 21.4% (88/411) of complicated pregnancies and 14.3% (52/363) of controls (OR 1.62, 95% CI 1.12-2.37). There were no differences in the fractions of endothelial cells, fibrin deposition, macrophages, and neutrophils when comparing normal and complicated pregnancies. In conclusion, FGR and pre-eclampsia are associated with T-cell infiltration of the placenta and placental pathological abnormalities.
CD8-Positive T-Lymphocytes, Endothelial Cells, Placenta, Humans, Fetal Growth Retardation, Pre-Eclampsia, Pregnancy, Infant, Newborn, Female
This study was supported by grants from the Medical Research Council (United Kingdom; MR/K021133/1) and the National Institute for Health Research (NIHR) Cambridge Biomedical Research Centre (Women’s Health theme). The funding sources had no involvement in study design, data collection, analysis or interpretation, manuscript preparation or deciding to submit the article for publication.
Cambridge University Hospitals NHS Foundation Trust (CUH) (146281)
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External DOI: https://doi.org/10.1113/jp279532
This record's URL: https://www.repository.cam.ac.uk/handle/1810/310199
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