Structure and Dynamics of GPCRs in Lipid Membranes: Physical Principles and Experimental Approaches.
Jones, Andrew JY
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Jones, A. J., Gabriel, F., Tandale, A., & Nietlispach, D. (2020). Structure and Dynamics of GPCRs in Lipid Membranes: Physical Principles and Experimental Approaches.. Molecules, 25 (20) https://doi.org/10.3390/molecules25204729
Over the past decade, the vast amount of information generated through structural and biophysical studies of GPCRs has provided unprecedented mechanistic insight into the complex signalling behaviour of these receptors. With this recent information surge, it has also become increasingly apparent that in order to reproduce the various effects that lipids and membranes exert on the biological function for these allosteric receptors, in vitro studies of GPCRs need to be conducted under conditions that adequately approximate the native lipid bilayer environment. In the first part of this review, we assess some of the more general effects that a membrane environment exerts on lipid bilayer-embedded proteins such as GPCRs. This is then followed by the consideration of more specific effects, including stoichiometric interactions with specific lipid subtypes. In the final section, we survey a range of different membrane mimetics that are currently used for in vitro studies, with a focus on NMR applications.
Humans, Membrane Lipids, Lipid Bilayers, Receptors, G-Protein-Coupled, Magnetic Resonance Spectroscopy, Biomimetics, Signal Transduction, Molecular Structure, Physical Phenomena
This work was supported by the Biotechnology and Biological Sciences Research Council (BB/S015892/1) (DN & AJYJ), German Academic Exchange Service (FG), Newnham College and a Trinity Henry-Barlow Scholarship (AT).
Biotechnology and Biological Sciences Research Council (BB/S015892/1)
External DOI: https://doi.org/10.3390/molecules25204729
This record's URL: https://www.repository.cam.ac.uk/handle/1810/311535
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