Aptamer-modified gold nanoparticles for rapid aggregation-based detection of inflammation: an optical assay for interleukin-6
Hutchinson, Peter J.
Carpenter, Keri L.H.
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Giorgi-Coll, S., Marín, M. J., Sule, O., Hutchinson, P. J., & Carpenter, K. L. (2019). Aptamer-modified gold nanoparticles for rapid aggregation-based detection of inflammation: an optical assay for interleukin-6. Microchimica Acta, 187 (1) https://doi.org/10.1007/s00604-019-3975-7
Abstract: A proof-of-concept aptamer-based optical assay is described for the determination of the immuno signalling molecule interleukin-6 (IL-6), a key marker of acute inflammation. The optical assay is based on the aggregation of gold nanoparticles (AuNP) coated in two complimentary “sandwich-style” aptamers, each with different IL-6 target moieties. IL-6 will recognise the complimentary aptamer pair and bind to it, thereby causing the aggregation of the corresponding functionalised nanoparticles. The aggregation of the AuNPs after exposure to IL-6 induces a visible colour change from red to pink, with a corresponding change in the absorption maximum from 520 to 540 nm. The change in the absorption maximum can be monitored visually, or by using a spectrophotometer or a plate reader. The optimal size and functionalisation of aptamer-coated AuNPs, and the potential assay formats were investigated using UV-vis spectrophotometry, transmission electron microscopy, and dynamic light scattering. The optical assay was applied for detecting mouse IL-6 in a mixed protein solution as a representative biological sample. The assay works in the 3.3 to 125 μg·mL−1 IL-6 concentration range, and the detection limit (at S/N = 3) is 1.95 μg·mL−1. This study was performed as a proof-of-concept demonstration of this versatile assay design, with a view to developing a similar assay for use in clinical samples in future. Graphical abstractSchematic representation of the aggregation of aptamer-functionalised nanoparticles in the presence of interleukin-6 (IL-6). The presence of mouse IL-6 in a mixed protein solution leads to a visible colour change, and a change in the absorption spectrum of the nanoparticles.
Original Paper, Cytokines, Colorimetry, Metal nanoparticles, Sepsis, Diagnostics, Point-of-care assay
National Institute for Health Research Brain Injury Healthcare Technology Co-operative (n/a)
National Institute for Health Research Biomedical Research Centre, Cambridge (Neuroscience Theme (n/a, n/a)
National Institute for Health Research Research Professorship (n/a)
Academy of Medical Sciences/Health Foundation Senior Surgical Scientist Fellowship (n/a)
National Institute for Health Research Biomedical Research Centre, Cambridge (n/a)
External DOI: https://doi.org/10.1007/s00604-019-3975-7
This record's URL: https://www.repository.cam.ac.uk/handle/1810/314464
Attribution 4.0 International (CC BY 4.0)
Licence URL: https://creativecommons.org/licenses/by/4.0/