Biophysics of high density nanometer regions extracted from super-resolution single particle trajectories: application to voltage-gated calcium channels and phospholipids.
Authors
Parutto, P
Heck, J
Heine, M
Holcman, D
Publication Date
2019-12-11Journal Title
Sci Rep
ISSN
2045-2322
Publisher
Springer Science and Business Media LLC
Volume
9
Issue
1
Language
en
Type
Article
This Version
VoR
Metadata
Show full item recordCitation
Parutto, P., Heck, J., Heine, M., & Holcman, D. (2019). Biophysics of high density nanometer regions extracted from super-resolution single particle trajectories: application to voltage-gated calcium channels and phospholipids.. Sci Rep, 9 (1) https://doi.org/10.1038/s41598-019-55124-8
Abstract
The cellular membrane is very heterogenous and enriched with high-density regions forming microdomains, as revealed by single particle tracking experiments. However the organization of these regions remain unexplained. We determine here the biophysical properties of these regions, when described as a basin of attraction. We develop two methods to recover the dynamics and local potential wells (field of force and boundary). The first method is based on the local density of points distribution of trajectories, which differs inside and outside the wells. The second method focuses on recovering the drift field that is convergent inside wells and uses the transient field to determine the boundary. Finally, we apply these two methods to the distribution of trajectories recorded from voltage gated calcium channels and phospholipid anchored GFP in the cell membrane of hippocampal neurons and obtain the size and energy of high-density regions with a nanometer precision.
Keywords
Article, /631/57/2266, /631/57/2265, /639/766/747, /639/705/1046, article
Identifiers
s41598-019-55124-8, 55124
External DOI: https://doi.org/10.1038/s41598-019-55124-8
This record's URL: https://www.repository.cam.ac.uk/handle/1810/314963
Rights
Attribution 4.0 International (CC BY 4.0)
Licence URL: https://creativecommons.org/licenses/by/4.0/
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