Trophectoderm mechanics direct epiblast shape upon embryo implantation.
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Weberling, A., & Zernicka-Goetz, M. (2021). Trophectoderm mechanics direct epiblast shape upon embryo implantation.. Cell reports, 34 (3), 108655. https://doi.org/10.1016/j.celrep.2020.108655
Implantation is a hallmark of mammalian embryogenesis during which embryos establish their contacts with the maternal endometrium, remodel, and undertake growth and differentiation. The mechanisms and sequence of events through which embryos change their shape during this transition are largely unexplored. Here, we show that the first extraembryonic lineage, the polar trophectoderm, is the key regulator for remodelling the embryonic epiblast. Loss of its function following immuno-surgery or inhibitor treatments prevents the epiblast shape transitions. In the mouse, the polar trophectoderm exerts physical force upon the epiblast causing it to transform from an oval into the cup shape. In human embryos, the polar trophectoderm behaves in the opposite manner, exerting a stretching force. By mimicking this stretching behaviour in mouse embryogenesis, we could direct the epiblast to adopt the disc-like shape characteristic of human embryos at this stage. Thus, the polar trophectoderm acts as a conserved regulator of epiblast shape.
European Research Council (RG77946), the Welcome Trust (207415/Z/17/Z), the EU Horizon 2020 Marie Sklodowska-Curie actions (ImageInLife,721537) and Open Philanthropy and Weston Haven grants at Caltech.
ECH2020 EUROPEAN RESEARCH COUNCIL (ERC) (669198)
European Commission Horizon 2020 (H2020) Marie Sk?odowska-Curie actions (721537)
Wellcome Trust (207415/Z/17/Z)
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External DOI: https://doi.org/10.1016/j.celrep.2020.108655
This record's URL: https://www.repository.cam.ac.uk/handle/1810/315701
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