Repeat DNA-PAINT suppresses background and non-specific signals in optical nanoscopy.
Authors
Kaufhold, William T
Lutz, Tobias
Publication Date
2021-01-21Journal Title
Nat Commun
ISSN
2041-1723
Publisher
Springer Science and Business Media LLC
Volume
12
Issue
1
Language
en
Type
Article
This Version
VoR
Metadata
Show full item recordCitation
Clowsley, A. H., Kaufhold, W. T., Lutz, T., Meletiou, A., Di Michele, L., & Soeller, C. (2021). Repeat DNA-PAINT suppresses background and non-specific signals in optical nanoscopy.. Nat Commun, 12 (1) https://doi.org/10.1038/s41467-020-20686-z
Description
Funder: ODNI | Intelligence Community Postdoctoral Research Fellowship Program (IC Postdoctoral Research Fellowship Program); doi: https://doi.org/10.13039/100011040
Abstract
DNA-PAINT is a versatile optical super-resolution technique relying on the transient binding of fluorescent DNA 'imagers' to target epitopes. Its performance in biological samples is often constrained by strong background signals and non-specific binding events, both exacerbated by high imager concentrations. Here we describe Repeat DNA-PAINT, a method that enables a substantial reduction in imager concentration, thus suppressing spurious signals. Additionally, Repeat DNA-PAINT reduces photoinduced target-site loss and can accelerate sampling, all without affecting spatial resolution.
Keywords
Article, /631/1647/245/2225, /631/1647/328/2238, /631/61, /13/51, /13/1, /14/63, /123, article
Sponsorship
EC | EU Framework Programme for Research and Innovation H2020 | H2020 Priority Excellent Science | H2020 European Research Council (H2020 Excellent Science - European Research Council) (ERC-STG No 851667 — NANOCELL)
RCUK | Engineering and Physical Sciences Research Council (EPSRC) (EP/N008235/1)
RCUK | Biotechnology and Biological Sciences Research Council (BBSRC) (BB/P026508/1, BB/T007176/1)
Identifiers
s41467-020-20686-z, 20686
External DOI: https://doi.org/10.1038/s41467-020-20686-z
This record's URL: https://www.repository.cam.ac.uk/handle/1810/316546
Rights
Attribution 4.0 International (CC BY 4.0)
Licence URL: https://creativecommons.org/licenses/by/4.0/
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