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dc.contributor.authorClowsley, Alexander H.
dc.contributor.authorKaufhold, William T.
dc.contributor.authorLutz, Tobias
dc.contributor.authorMeletiou, Anna
dc.contributor.authorDi Michele, Lorenzo
dc.contributor.authorSoeller, Christian
dc.date.accessioned2021-01-21T17:24:23Z
dc.date.available2021-01-21T17:24:23Z
dc.date.issued2021-01-21
dc.date.submitted2020-04-28
dc.identifier.others41467-020-20686-z
dc.identifier.other20686
dc.identifier.urihttps://www.repository.cam.ac.uk/handle/1810/316546
dc.descriptionFunder: ODNI | Intelligence Community Postdoctoral Research Fellowship Program (IC Postdoctoral Research Fellowship Program); doi: https://doi.org/10.13039/100011040
dc.description.abstractAbstract: DNA-PAINT is a versatile optical super-resolution technique relying on the transient binding of fluorescent DNA ‘imagers’ to target epitopes. Its performance in biological samples is often constrained by strong background signals and non-specific binding events, both exacerbated by high imager concentrations. Here we describe Repeat DNA-PAINT, a method that enables a substantial reduction in imager concentration, thus suppressing spurious signals. Additionally, Repeat DNA-PAINT reduces photoinduced target-site loss and can accelerate sampling, all without affecting spatial resolution.
dc.languageen
dc.publisherNature Publishing Group UK
dc.rightsAttribution 4.0 International (CC BY 4.0)en
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/en
dc.subjectArticle
dc.subject/631/1647/245/2225
dc.subject/631/1647/328/2238
dc.subject/631/61
dc.subject/13/51
dc.subject/13/1
dc.subject/14/63
dc.subject/123
dc.subjectarticle
dc.titleRepeat DNA-PAINT suppresses background and non-specific signals in optical nanoscopy
dc.typeArticle
dc.date.updated2021-01-21T17:24:22Z
prism.issueIdentifier1
prism.publicationNameNature Communications
prism.volume12
dc.identifier.doi10.17863/CAM.63655
dcterms.dateAccepted2020-12-10
rioxxterms.versionofrecord10.1038/s41467-020-20686-z
rioxxterms.versionVoR
rioxxterms.licenseref.urihttp://creativecommons.org/licenses/by/4.0/
dc.contributor.orcidClowsley, Alexander H. [0000-0001-9318-1557]
dc.contributor.orcidMeletiou, Anna [0000-0003-1167-0555]
dc.contributor.orcidDi Michele, Lorenzo [0000-0002-1458-9747]
dc.contributor.orcidSoeller, Christian [0000-0002-9302-2203]
dc.identifier.eissn2041-1723
pubs.funder-project-idEC | EU Framework Programme for Research and Innovation H2020 | H2020 Priority Excellent Science | H2020 European Research Council (H2020 Excellent Science - European Research Council) (ERC-STG No 851667 — NANOCELL)
pubs.funder-project-idRCUK | Engineering and Physical Sciences Research Council (EPSRC) (EP/N008235/1)
pubs.funder-project-idRCUK | Biotechnology and Biological Sciences Research Council (BBSRC) (BB/P026508/1, BB/T007176/1)


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Attribution 4.0 International (CC BY 4.0)
Except where otherwise noted, this item's licence is described as Attribution 4.0 International (CC BY 4.0)