The intersection of DNA replication with antisense 3' RNA processing in <i>Arabidopsis FLC</i> chromatin silencing.
Proceedings of the National Academy of Sciences of the United States of America
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Baxter, C. L., Šviković, S., Sale, J. E., Dean, C., & Costa, S. (2021). The intersection of DNA replication with antisense 3' RNA processing in <i>Arabidopsis FLC</i> chromatin silencing.. Proceedings of the National Academy of Sciences of the United States of America, 118 (28)https://doi.org/10.1073/pnas.2107483118
Funder: Wellcome Trust
How noncoding transcription influences chromatin states is still unclear. The <i>Arabidopsis</i> floral repressor gene <i>FLC</i> is quantitatively regulated through an antisense-mediated chromatin silencing mechanism. The <i>FLC</i> antisense transcripts form a cotranscriptional R-loop that is dynamically resolved by RNA 3' processing factors (FCA and FY), and this is linked to chromatin silencing. Here, we investigate this silencing mechanism and show, using single-molecule DNA fiber analysis, that FCA and FY are required for unimpeded replication fork progression across the <i>Arabidopsis</i> genome. We then employ the chicken DT40 cell line system, developed to investigate sequence-dependent replication and chromatin inheritance, and find that <i>FLC</i> R-loop sequences have an orientation-dependent ability to stall replication forks. These data suggest a coordination between RNA 3' processing of antisense RNA and replication fork progression in the inheritance of chromatin silencing at <i>FLC</i>.
Arabidopsis, Transcription, DNA replication, Chromatin silencing, Chicken Dt40
Biotechnology and Biological Sciences Research Council (BB/P013511/1)
European Research Council (833254, 210654)
External DOI: https://doi.org/10.1073/pnas.2107483118
This record's URL: https://www.repository.cam.ac.uk/handle/1810/326791
Attribution 4.0 International
Licence URL: https://creativecommons.org/licenses/by/4.0/