Ultrahigh-throughput enzyme screening in microfluidic droplets
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Journal Title
Trends in Biochemical Sciences
Publisher
Elsevier
Type
Article
This Version
AM
Metadata
Show full item recordCitation
Hollfelder, F., Aleku, G., & Gantz, M. Ultrahigh-throughput enzyme screening in microfluidic droplets. Trends in Biochemical Sciences https://doi.org/10.17863/CAM.78114
Abstract
Biocatalysis is an environmentally friendly, cost-efficient alternative to chemocatalytic synthesis. Yet improvements in specificity and efficiency are needed, and have to be generated quickly to meet the fast pace of product development in industry. This ambition is met by droplet microfluidics, a powerful tool to scan vast libraries at kHz speed with >107/day throughput. Instead of μL robotic liquid handling systems, pL water-in-oil emulsion droplets are generated in PDMS chips replacing the use of plasticware.
Sponsorship
Leverhulme
Funder references
BBSRC (BB/T003545/1)
Embargo Lift Date
2024-11-15
Identifiers
This record's DOI: https://doi.org/10.17863/CAM.78114
This record's URL: https://www.repository.cam.ac.uk/handle/1810/330669
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