The genetic and epigenetic landscape of the Arabidopsis centromeres.
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Authors
Naish, Matthew
Alonge, Michael
Tock, Andrew J
Abramson, Bradley W
Schmücker, Anna
Mandáková, Terezie
Jamge, Bhagyshree
Lambing, Christophe
Kuo, Pallas
Yelina, Natasha
Hartwick, Nolan
Colt, Kelly
Smith, Lisa M
Ton, Jurriaan
Kakutani, Tetsuji
Martienssen, Robert A
Schneeberger, Korbinian
Lysak, Martin A
Berger, Frédéric
Bousios, Alexandros
Michael, Todd P
Schatz, Michael C
Henderson, Ian R
Publication Date
2021-11-12Journal Title
Science
ISSN
0036-8075
Publisher
American Association for the Advancement of Science (AAAS)
Volume
374
Issue
6569
Number
7489
Language
eng
Type
Article
This Version
AM
Metadata
Show full item recordCitation
Naish, M., Alonge, M., Wlodzimierz, P., Tock, A. J., Abramson, B. W., Schmücker, A., Mandáková, T., et al. (2021). The genetic and epigenetic landscape of the Arabidopsis centromeres.. Science, 374 (6569. 7489) https://doi.org/10.1126/science.abi7489
Abstract
Centromeres attach chromosomes to spindle microtubules during cell division and, despite this conserved role, show paradoxically rapid evolution and are typified by complex repeats. We used longread sequencing to generate the Col-CEN Arabidopsis thaliana genome assembly that resolves all five centromeres. The centromeres consist of megabase-scale tandemly repeated satellite arrays, which support CENH3 occupancy and are densely DNA methylated, with satellite variants private to each chromosome. CENH3 preferentially occupies satellites that show least divergence and occur in higherorder repeats. The centromeres are invaded by ATHILA retrotransposons, which disrupt genetic and epigenetic organization. Centromeric crossover recombination is suppressed, yet low levels of meiotic DSBs occur that are regulated by DNA methylation. We propose that Arabidopsis centromeres are evolving via cycles of satellite homogenization and retrotransposon-driven diversification.
Keywords
Arabidopsis, Centromere, Chromosomes, Plant, DNA Methylation, DNA, Satellite, Epigenesis, Genetic, Evolution, Molecular, Genome, Plant, Histones, Meiosis, Recombination, Genetic, Retroelements, Sequence Analysis, DNA
Sponsorship
BBSRC grants BB/S006842/1, BB/S020012/1 and BB/V003984/1.
Funder references
Isaac Newton Trust (1026(ab))
Biotechnology and Biological Sciences Research Council (BB/N007557/1)
European Research Council (681987)
Biotechnology and Biological Sciences Research Council (BB/S006842/1)
Biotechnology and Biological Sciences Research Council (BB/S020012/1)
Leverhulme Trust (RPG-2019-259)
BBSRC (BB/V003984/1)
BBSRC (BB/T004282/1)
Identifiers
External DOI: https://doi.org/10.1126/science.abi7489
This record's URL: https://www.repository.cam.ac.uk/handle/1810/330969
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