The genetic and epigenetic landscape of the Arabidopsis centromeres.
Abramson, Bradley W
Smith, Lisa M
Martienssen, Robert A
Lysak, Martin A
Michael, Todd P
Schatz, Michael C
American Association for the Advancement of Science (AAAS)
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Naish, M., Alonge, M., Wlodzimierz, P., Tock, A., Abramson, B. W., Schmücker, A., Mandáková, T., et al. (2021). The genetic and epigenetic landscape of the Arabidopsis centromeres.. Science, 374 (6569. 7489) https://doi.org/10.1126/science.abi7489
Centromeres attach chromosomes to spindle microtubules during cell division and, despite this conserved role, show paradoxically rapid evolution and are typified by complex repeats. We used longread sequencing to generate the Col-CEN Arabidopsis thaliana genome assembly that resolves all five centromeres. The centromeres consist of megabase-scale tandemly repeated satellite arrays, which support CENH3 occupancy and are densely DNA methylated, with satellite variants private to each chromosome. CENH3 preferentially occupies satellites that show least divergence and occur in higherorder repeats. The centromeres are invaded by ATHILA retrotransposons, which disrupt genetic and epigenetic organization. Centromeric crossover recombination is suppressed, yet low levels of meiotic DSBs occur that are regulated by DNA methylation. We propose that Arabidopsis centromeres are evolving via cycles of satellite homogenization and retrotransposon-driven diversification.
Arabidopsis, Centromere, Chromosomes, Plant, DNA Methylation, DNA, Satellite, Epigenesis, Genetic, Evolution, Molecular, Genome, Plant, Histones, Meiosis, Recombination, Genetic, Retroelements, Sequence Analysis, DNA
BBSRC grants BB/S006842/1, BB/S020012/1 and BB/V003984/1.
Isaac Newton Trust (1026(ab))
Biotechnology and Biological Sciences Research Council (BB/N007557/1)
European Research Council (681987)
Biotechnology and Biological Sciences Research Council (BB/S006842/1)
Biotechnology and Biological Sciences Research Council (BB/S020012/1)
Leverhulme Trust (RPG-2019-259)
External DOI: https://doi.org/10.1126/science.abi7489
This record's URL: https://www.repository.cam.ac.uk/handle/1810/330969
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