Synergistic insights into human health from aptamer- and antibody-based proteomic profiling
Authors
Wheeler, Eleanor
Kerrison, Nicola D.
Oerton, Erin
Publication Date
2021-11-24Journal Title
Nature Communications
Publisher
Nature Publishing Group UK
Volume
12
Issue
1
Language
en
Type
Article
This Version
VoR
Metadata
Show full item recordCitation
Pietzner, M., Wheeler, E., Carrasco-Zanini, J., Kerrison, N. D., Oerton, E., Koprulu, M., Luan, J., et al. (2021). Synergistic insights into human health from aptamer- and antibody-based proteomic profiling. Nature Communications, 12 (1) https://doi.org/10.1038/s41467-021-27164-0
Abstract
Abstract: Affinity-based proteomics has enabled scalable quantification of thousands of protein targets in blood enhancing biomarker discovery, understanding of disease mechanisms, and genetic evaluation of drug targets in humans through protein quantitative trait loci (pQTLs). Here, we integrate two partly complementary techniques—the aptamer-based SomaScan® v4 assay and the antibody-based Olink assays—to systematically assess phenotypic consequences of hundreds of pQTLs discovered for 871 protein targets across both platforms. We create a genetically anchored cross-platform proteome-phenome network comprising 547 protein–phenotype connections, 36.3% of which were only seen with one of the two platforms suggesting that both techniques capture distinct aspects of protein biology. We further highlight discordance of genetically predicted effect directions between assays, such as for PILRA and Alzheimer’s disease. Our results showcase the synergistic nature of these technologies to better understand and identify disease mechanisms and provide a benchmark for future cross-platform discoveries.
Keywords
Article, /631/45/612/1221, /631/208/729/743, /631/1647/2067, /692/699, /82/79, /45/43, article
Identifiers
s41467-021-27164-0, 27164
External DOI: https://doi.org/10.1038/s41467-021-27164-0
This record's URL: https://www.repository.cam.ac.uk/handle/1810/331034
Rights
Licence:
http://creativecommons.org/licenses/by/4.0/
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