Recombinant expression of insoluble enzymes in Escherichia coli: a systematic review of experimental design and its manufacturing implications.
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Publication Date
2021-10-30Journal Title
Microb Cell Fact
ISSN
1475-2859
Publisher
Springer Science and Business Media LLC
Volume
20
Issue
1
Language
eng
Type
Article
This Version
VoR
Metadata
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Mital, S., Christie, G., & Dikicioglu, D. (2021). Recombinant expression of insoluble enzymes in Escherichia coli: a systematic review of experimental design and its manufacturing implications.. Microb Cell Fact, 20 (1) https://doi.org/10.1186/s12934-021-01698-w
Abstract
Recombinant enzyme expression in Escherichia coli is one of the most popular methods to produce bulk concentrations of protein product. However, this method is often limited by the inadvertent formation of inclusion bodies. Our analysis systematically reviews literature from 2010 to 2021 and details the methods and strategies researchers have utilized for expression of difficult to express (DtE), industrially relevant recombinant enzymes in E. coli expression strains. Our review identifies an absence of a coherent strategy with disparate practices being used to promote solubility. We discuss the potential to approach recombinant expression systematically, with the aid of modern bioinformatics, modelling, and 'omics' based systems-level analysis techniques to provide a structured, holistic approach. Our analysis also identifies potential gaps in the methods used to report metadata in publications and the impact on the reproducibility and growth of the research in this field.
Keywords
Inclusion bodies, Solubility, systems biology, Escherichia Coli, Difficult To Express Enzymes, Recombinant Industrial Enzymes
Sponsorship
None
Identifiers
PMC8557517, 34717620
External DOI: https://doi.org/10.1186/s12934-021-01698-w
This record's URL: https://www.repository.cam.ac.uk/handle/1810/332211
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