Recombinant expression of insoluble enzymes in Escherichia coli: a systematic review of experimental design and its manufacturing implications.
Microb Cell Fact
Springer Science and Business Media LLC
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Mital, S., Christie, G., & Dikicioglu, D. (2021). Recombinant expression of insoluble enzymes in Escherichia coli: a systematic review of experimental design and its manufacturing implications.. Microb Cell Fact, 20 (1) https://doi.org/10.1186/s12934-021-01698-w
Recombinant enzyme expression in Escherichia coli is one of the most popular methods to produce bulk concentrations of protein product. However, this method is often limited by the inadvertent formation of inclusion bodies. Our analysis systematically reviews literature from 2010 to 2021 and details the methods and strategies researchers have utilized for expression of difficult to express (DtE), industrially relevant recombinant enzymes in E. coli expression strains. Our review identifies an absence of a coherent strategy with disparate practices being used to promote solubility. We discuss the potential to approach recombinant expression systematically, with the aid of modern bioinformatics, modelling, and 'omics' based systems-level analysis techniques to provide a structured, holistic approach. Our analysis also identifies potential gaps in the methods used to report metadata in publications and the impact on the reproducibility and growth of the research in this field.
Inclusion bodies, Solubility, systems biology, Escherichia Coli, Difficult To Express Enzymes, Recombinant Industrial Enzymes
External DOI: https://doi.org/10.1186/s12934-021-01698-w
This record's URL: https://www.repository.cam.ac.uk/handle/1810/332211
Attribution 4.0 International
Licence URL: https://creativecommons.org/licenses/by/4.0/