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dc.contributor.authorMital, Suraj
dc.contributor.authorChristie, Graham
dc.contributor.authorDikicioglu, Duygu
dc.date.accessioned2022-01-06T12:55:42Z
dc.date.available2022-01-06T12:55:42Z
dc.date.issued2021-10-30
dc.identifier.issn1475-2859
dc.identifier.otherPMC8557517
dc.identifier.other34717620
dc.identifier.urihttps://www.repository.cam.ac.uk/handle/1810/332211
dc.description.abstractRecombinant enzyme expression in Escherichia coli is one of the most popular methods to produce bulk concentrations of protein product. However, this method is often limited by the inadvertent formation of inclusion bodies. Our analysis systematically reviews literature from 2010 to 2021 and details the methods and strategies researchers have utilized for expression of difficult to express (DtE), industrially relevant recombinant enzymes in E. coli expression strains. Our review identifies an absence of a coherent strategy with disparate practices being used to promote solubility. We discuss the potential to approach recombinant expression systematically, with the aid of modern bioinformatics, modelling, and 'omics' based systems-level analysis techniques to provide a structured, holistic approach. Our analysis also identifies potential gaps in the methods used to report metadata in publications and the impact on the reproducibility and growth of the research in this field.
dc.description.sponsorshipNone
dc.languageeng
dc.publisherSpringer Science and Business Media LLC
dc.rightsAttribution 4.0 International
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/
dc.sourceessn: 1475-2859
dc.sourcenlmid: 101139812
dc.subjectInclusion bodies
dc.subjectSolubility
dc.subjectsystems biology
dc.subjectEscherichia Coli
dc.subjectDifficult To Express Enzymes
dc.subjectRecombinant Industrial Enzymes
dc.titleRecombinant expression of insoluble enzymes in Escherichia coli: a systematic review of experimental design and its manufacturing implications.
dc.typeArticle
dc.date.updated2022-01-06T12:55:41Z
prism.issueIdentifier1
prism.publicationNameMicrob Cell Fact
prism.volume20
dc.identifier.doi10.17863/CAM.79657
dcterms.dateAccepted2021-10-22
rioxxterms.versionofrecord10.1186/s12934-021-01698-w
rioxxterms.versionVoR
rioxxterms.licenseref.urihttps://creativecommons.org/licenses/by/4.0/
dc.contributor.orcidDikicioglu, Duygu [0000-0002-3018-4790]
dc.identifier.eissn1475-2859
cam.issuedOnline2021-10-30


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Attribution 4.0 International
Except where otherwise noted, this item's licence is described as Attribution 4.0 International