Assessing Phototoxicity in a Mammalian Cell Line: How Low Levels of Blue Light Affect Motility in PC3 Cells.
Authors
Alghamdi, Rana A
Exposito-Rodriguez, Marino
Mullineaux, Philip M
Brooke, Greg N
Laissue, Philippe P
Publication Date
2021Journal Title
Front Cell Dev Biol
ISSN
2296-634X
Publisher
Frontiers Media SA
Volume
9
Language
en
Type
Article
This Version
VoR
Metadata
Show full item recordCitation
Alghamdi, R. A., Exposito-Rodriguez, M., Mullineaux, P. M., Brooke, G. N., & Laissue, P. P. (2021). Assessing Phototoxicity in a Mammalian Cell Line: How Low Levels of Blue Light Affect Motility in PC3 Cells.. Front Cell Dev Biol, 9 https://doi.org/10.3389/fcell.2021.738786
Abstract
Phototoxicity is a significant constraint for live cell fluorescence microscopy. Excessive excitation light intensities change the homeostasis of the observed cells. Erroneous and misleading conclusions may be the problematic consequence of observing such light-induced pathophysiology. In this study, we assess the effect of blue light, as commonly used for GFP and YFP excitation, on a motile mammalian cell line. Tracking PC3 cells at different light doses and intensities, we show how motility can be used to reliably assess subtle positive and negative effects of illumination. We further show that the effects are a factor of intensity rather than light dose. Mitotic delay was not a sensitive indicator of phototoxicity. For early detection of the effect of blue light, we analysed the expression of genes involved in oxidative stress. This study addresses the need for relatively simple and sensitive methods to establish a dose-response curve for phototoxicity in mammalian cell line models. We conclude with a working model for phototoxicity and recommendations for its assessment.
Keywords
Cell and Developmental Biology, fluorescence, microscopy—light, live imaging, reactive oxygen species (ROS), light intensity (irradiance)
Identifiers
738786
External DOI: https://doi.org/10.3389/fcell.2021.738786
This record's URL: https://www.repository.cam.ac.uk/handle/1810/332414
Rights
Licence:
http://creativecommons.org/licenses/by/4.0/
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